摘要
为了探讨萘丁美酮的诱变性和致癌性，选用Ａｍｅｓ试验（采用ＴＡ９７、ＴＡ９８、ＴＡ１００、ＴＡ１０２４个标准菌株）、小鼠骨髓微核试验、体外培养的ＣＨＬ细胞染色体畸变分析、小鼠睾丸生殖细胞染色体畸变分析和叙利亚地鼠胚胎细胞（ＳＨＥ）转化试验进行了研究。结果所有试验均为阴性结果，未见萘丁美酮有诱变性和致癌性。
To study the mutagenicity and carcinogenicity of Nabumetone,we conducted Ames test(TA 97 ,TA 98 ,TA 100 ,TA 102 ),micronucleus test(MN) in mice marrow,chromosomal aberration assay(CA) in CHL cells in vitro,CA in germ cells from testes of mice,and cell transformation test of Syrian hamster embryo(SHE) cells.The maximum concentration was 500μg/plate in Ames test with and without S 9 mix.The mice were treated orally(gavage) daily for 4 days in 3 doses in which the maximum dose was 60% LD 50 and sampled at the 5th day in MN.The maximum concentration was the dose that the growth of 50% of cells was inhibited in CA of CHL.Cells were harvested after recultured for 18 hours in fresh medium after treatment for 6 hours in the test with S 9 mix,and after treatment for 24 or 48 hours in the without S 9 mix.The mice were treated orally(gavage) daily for 5 days in 3 doses in which the maximum dose was 1/4 LD 50 and sampled at the 6th day in CA of germ cells from testes of mice.2μg/ml was chosen as the maximum concentration in the cell transformation test of SHE cells,and result was observed after treatment for 9 days,All the tests obtained the same negative result as that reported by other investigators.
出处
《华西医科大学学报》
CSCD
1997年第2期174-178,共5页
Journal of West China University of Medical Sciences
关键词
萘丁美酮
诱变性
致癌性
Nabumetone Mutagenicity Carcinogenicity
