摘要
作者用等电聚焦免疫印迹法（ＩＥＦＩＢ）对人类精液（斑）、阴道液及混合斑中转铁蛋白（Ｔｆ）亚型进行了检测，结果发现８份精液和室温下保存３２周的精斑与同一个体血清的Ｔｆ亚型相同，用ＩＥＦＩＢ法未测出阴道液及含少量精液的混合斑中Ｔｆ的亚型。结果提示：Ｔｆ在精斑中稳定，ＩＥＦＩＢ法可检测强奸案的精斑和含精液较多的混合斑中Ｔｆ的亚型。
Transferrin (T f) is an important genetic marker for personal identification in forensic science.We examined the subtypes in human semen,semen stain,vaginal fluid and mixed stain using isoeleetric focusing and immunoblotting method(IEFIB).The results showed that.T f subtypes of 8 semen and semen stain samples stored at room temperature for up to 32 weeks were the same as those in sera. T f subtypes of 17 vaginal fluid and 4 mixed stain containing few semen were not detected. T f Subtyping of 1 mixed stain containing enoregh semen was successful by using IEFIB.These results indicate that T f is stable in semen stain.The T f subtyping in semen stain and mixed stain containing enough semen can be carried out by using IEFIB method.
出处
《华西医科大学学报》
CSCD
1997年第2期160-162,共3页
Journal of West China University of Medical Sciences
关键词
Tf亚型
精液
精斑
阴道液
混合斑
IEFIB
Transferrin subtytping Isoelestectric focusing immunoblotting Semen(stain) Vaginal fluid Mixed stain
