摘要
用扩增产物片段长度多态性分析方法测出人类血痕、精液、精液与阴道分泌液混合斑、唾液（斑）与毛发的ＡｐｏＢ３′ＶＮＴＲ位点的ＤＮＡ遗传多态性，探讨了不同保存温度血痕中ＡｐｏＢ３′位点ＤＮＡ多态性的可检出时限，发现室温保存１５周以内，－２０℃保存两年以内的血痕均可测出ＡｐｏＢ３′位点的ＤＮＡ多态性。用该法作３４例亲子鉴定，其中８例否定了错误被控父亲，８例中６例ＡｐｏＢ３′位点参与了否定。对３例强奸案的混合斑进行个人鉴定，否定了２例的嫌疑对象。
Amp FLP analyses of Apo B 3′ VNTR locus in human blood stain, saliva(stain),semen, mixed stain and hair have been carried out. The genotypings have been achieved. The genotypes of Apo B 3′ locus can be detected accurately in blood stains kept at room temperature within 15 weeks and at -20℃ within two years. In a series of paternity testing on 34 cases, 8 false alleged fathers were excluded; of them, 6 were excluded by Apo B 3′ locus alone or by Apo B 3′ locus combined with other genetic markers. 3 rape cases were investigated; 2 suspects were excluded.
出处
《华西医科大学学报》
CSCD
1997年第2期149-153,共5页
Journal of West China University of Medical Sciences
基金
卫生部科学研究基金
纽约中华医学基金
