摘要
目的探讨细胞外信号调节激酶在哮喘大鼠气道中表达变化及其对气道平滑肌细胞增殖的影响。观察细胞外信号调节激酶是否参与了哮喘气道重构这一病理过程。方法18只6周龄雄性wistar大鼠随机分为对照组、哮喘组、地塞米松干预组各6只。以腹腔注射10%卵蛋白和1%卵蛋白雾化吸入复制慢性哮喘模型。干预组在每次激发前给予地塞米松干预。用免疫组化与原位杂交法检测p-ERK1/2及ERK2 mRNA在不同大鼠肺组织的表达程度,采用图像分析系统进行图象分析。结果(1)哮喘模型组气道壁面积和平滑肌厚度较对照组和干预组显著增加(P<0.05)。(2)哮喘组p-ERK1/2及ERK2 mRNA在大鼠肺组织的表达程度较对照组和干预组显著增加(P<0.05)。(3)直线相关性分析显示,哮喘组气道壁面积和平滑肌厚度与大鼠肺组织中p-ERK1/2表达水平呈正相关(分别为r=0.858,r=0.848,P均<0.05),哮喘组气道壁面积和平滑肌厚度与大鼠肺组织中ERK2 mRNA表达水平呈正相关,(分别为r=0.918,r=0.860,P均<0.05)。结论哮喘大鼠肺组织p-ERK1/2及ERK2 mRNA表达上调,并与气道重构密切相关,该结果提示细胞外信号调节激酶可能参与了气道重构中平滑肌的增殖过程。
Objective To investigate the correlation of the airway smooth muscle cells proliferation with the expression of ERK in the lung tissues of asthmatic rats. Methods Eighteen wistar rats were randomly divided into three groups： the control group, the asthma model group and the dexamethasone group . The asthma model was established by sensitization and challenged with ovalbumin, and the rats in the dethamethasone group inhaled dexamethasone before the challenge. Immunohistocbemistry and In situ hybridization were used to measure the level of p-ERK and ERK2 mRNA in the rats＇ lung tissues. The wall area and the thickness of the airway smooth muscle was estimated by a computerized digital image analyzer. Results （ 1 ） The wall area and the thickness of the airway smooth muscle in the asthma group was higher than in the other groups （P 〈 0.05）. （2） The expression of p-ERK1/2 and ERK2 mRNA in the lung tissues of the asthma model group bighter than that in the other groups （ P 〈 0.05）. （3） p-ERK1/2 expressions were positively correlated with the wall area and the airway smooth muscle thickness（ r = 0.858,r = 0.848, respectively）. （4） ERK2 mRNA expressions were positively correlated with the wall area and the airway smooth muscle thickness（ r = 0.918, r = 0.860, respectively）. Conclusion The expressions of p-ERK1/2 and ERK2 mRNA were increased in the lung tissues of asthmatic rats and positively correlated with the proliferation of airway smooth muscle. ERK may take part in the process of airway smooth muscle proliferation in the airway remodeling of asthma.
出处
《中国比较医学杂志》
CAS
2008年第3期14-18,84,共6页
Chinese Journal of Comparative Medicine
基金
2005年山西高校科技研究开发项目(20051001)
关键词
哮喘
气道重构
细胞外信号调节激酶
Asthma
Airwaywall remodeling
Extracellular signal regulated kinase
