摘要
目的探讨神经元烟碱受体(neuronal nicotinic acetyl-choline receptors,nnAChRs)与异氟烷、七氟烷催眠和镇痛作用的关系。方法建立小鼠催眠、镇痛模型后,在催醒、热板和扭体实验中分别观察侧脑室(intracerebroventricular,icv)或鞘内(intrathecal,it)注射不同剂量烟碱(nicotine,N)对小鼠睡眠时间(sleeping time,ST)、热板法痛阈(pain threshold in hot-plate test,HPPT)和扭体次数的影响。结果催醒实验中,烟碱10、20、40μgicv能够剂量依赖性地缩短异氟烷、七氟烷催眠小鼠的ST(P<0·05,P<0·01);热板实验中,烟碱5、10、15μgit对清醒小鼠HPPT没有影响(P>0·05),但能够剂量依赖性地减少异氟烷、七氟烷镇痛小鼠的HPPT(P<0·05,P<0·01);扭体实验中,皮下注射镇痛剂量的异氟烷、七氟烷后均能引起小鼠的扭体次数减少(P<0·01),但烟碱5、10、15μgit对异氟烷、七氟烷镇痛小鼠的扭体次数的影响差异均无显著性(P>0·05)。结论nnAChRs是异氟烷、七氟烷催眠作用的重要靶位;也是异氟烷、七氟烷抗热刺激伤害的重要靶位,但与其抗化学内脏痛作用关系不大。
Ahn To investigate the relationship between neuronal nicotinic acetylcholine receptors （nnAChRs） and the hypnotic and analgesic effects of isoflurane and sevoflurane. Methods After the establishment of the mice model of hypnosis and analgesia by intraperitoneal injection （ip）of appropriate doses of isoflurane or sevoflurane, the different doses of nicotine were injected intra-cerebroventricularly （icy） or intrathecally（it） and then their effects were observed on the sleeping time（ ST）, the pain threshold in hot-plate test （HPPT） and writhing times by using acetic acid-induced writhing test. Results In awaken test, nico- tine 10,20,40 μg（icy） could significantly decrease ST of the mice treated with isoflurane or sevoflurane （P 〈 0. 05 or P 〈0. 01 ） ;in hot-plate test, nicotine 5,10,15 μg （it） did not affect the HPPT in conscious mice （P 〉0. 05 ）, in contrast, nicotine 5,10,15 μg （it） could significantly and dose-dependently decrease the HPPT of the mice treated with isoflurane or sevoflurane （ P 〈 0.05 or P〈0.01 ）. In acetic acid-induced writhingtest, writhing times inhibition induced by sc administered isoflurane or sevoflurane was not affected by the nicotine 5, 10, 15 μg （ P 〉 0. 05 ）. Conclusions nnAChRs may be important targets for the hypnotic effects and analgesic effects on thermal-induced nociception but not chemcial-induced nociception of isoflu-rane and sevoflurane.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第3期318-322,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30471657
39970715)
江苏省自然科学基金资助项目(NoBK2001143)
