摘要
目的探讨姜黄素对人脐静脉内皮细胞基质金属蛋白酶-9(MMP-9)表达的影响。方法进行人脐静脉内皮细胞的体外培养和鉴定。加入不同浓度的姜黄素处理细胞24h。采用免疫细胞化学染色法和ELISA法测定细胞MMP-9的表达。结果不同浓度的姜黄素(6.25,12.5,25,50,100μg/mL)作用人脐静脉内皮细胞24h后均能明显抑制细胞MMP-9的表达,并且随着浓度的升高抑制作用明显增强。结论姜黄素能明显降低人脐静脉内皮细胞MMP-9的表达。
Objective To investigate the effect of curcumine on the expression of matrix metalloproteinase - 9 （MMP - 9） in human umbilical vein endothelial cell （HUVEC）. Methods HUVEC were cultured and identified in vitro. After cultured with curcumine at different concentration for 24 hours, the expression of MMP - 9 in HUVEC was detected by cytoimmunochemical staining method and ELISA. Results All concentrations Of curcumine can restrain the expression of HUVEC after co - culturing with the cells for 24 hours. The inhibitory effect got stronger with the increase of the concentration of curcumine. Conclusion The curcumine can depress the expression of matrix metalloproteinase - 9 in HUVEC obviously.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第2期97-99,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
姜黄素
人脐静脉内皮细胞
基质金属蛋白酶9
Curcumine
Human umbilical vein endothelial cell
Matrix metalloproteinase - 9
