摘要
目的:探讨mtDNA4977bp缺失用于肿瘤细胞辐射敏感性检测的可行性。方法:分别采用MTT法和巢式PCR法测定人肿瘤细胞株—肝癌细胞(HepG2)、食管癌细胞(EC-9706)和乳腺癌细胞(MCF-7)不同剂量γ射线照射后的存活分数(SF)和mtDNA4977bp缺失率。结果:MTT法:2Gy、4Gy和8Gy照射后,HepG2和EC-9706的SF显著低于MCF-7,表明HepG2和EC-9706细胞具有更高的辐射敏感性,HepG2细胞照射后的SF略低于EC-9706细胞,但统计学差异不显著。PCR法:1Gy和4Gy照射后3种肿瘤细胞mtDNA4977bp缺失率无显著性差异。8Gy照射后HepG2和EC-9706 mtDNA4977bp缺失进一步增加,而MCF-7的缺失率下降,显著低于HepG2与EC-9706细胞的缺失率,但HepG2和EC-9706细胞的缺失率无统计学差异,提示HepG2和EC-9706细胞的辐射敏感性高于MCF-7细胞,这与MTT法测定结果相符。结论:测定mtDNA4977bp缺失作为快速、简便的检测方法,有望用于肿瘤细胞辐射敏感性预测。
Objective ： To investigate the feasibility of determining radiosensitivity of tumor cell lines in vitro using the assay of mtDNA4977bp deletion . Methods： The surviving fraction（SF）and the ratio of mtDNA4977bp deletion were determined by MTT assay and nested PCR technique respectively after tumor cells of HepG2、 MCF-7 and EC-9706 were irradiated by various doses of 7-ray radiation from 0Gy to 8Gy. Results： MTT assay：The SF of HepG2 and EC-9706 after irradiated by 2Gy.4Gy and 8Gy radiation was lower significantly than that of MCF-7,which showed radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7. But there was no statistical difference of SF between HepG2 and EC-9706. PCR method ：The differences on mtDNA 4977bp deletion in mitochondrial DNA between HepG2, EC-9706 and MCF-7 were not significant after 1Gy and 4Gy 7 radiation. The ratio of 4977bp deletion in mitochondrial DNA of HepG2 and EC-9706 increased while MCF-7 decreased after exposure to 8Gy radiation. The ratio of mtDNA 4977bp deletion of HepG2 and EC-9706 was higher significantly than that of MCF-7 after irradiated by 8Gy,which implied radiosensitivity of HepG2 and EC-9706 was higher than that of MCF-7,which is consistent with the result from MMT assay. But the ratio of mtDNA 4977bp deletion was not statistically different between HepG2 and EC-9706. Conclusion： The method of mtDNA4977bp deletion may be used as a marker to predict radiosensitivity of tumor cells.
出处
《陕西医学杂志》
CAS
北大核心
2008年第3期262-265,共4页
Shaanxi Medical Journal
基金
中国医学科学院放射医学研究所青年创新探索基金(S617)
天津市自然科学基金(07JCYBJC09200)
