摘要
在制备壳聚糖衍生物N，N，N-三甲基壳聚糖盐酸盐（TMC）的基础上，通过将两种性质相反的电解质溶液进行共混，制备了一种新型的TMC／CMC（羧甲基壳聚糖）纳米载药颗粒体系。用激光散射仪和透射电镜表征了空白颗粒和载药颗粒的粒径、粒径分布、Zeta电位和形态结构。载药体系纳米颗粒的粒径在200～600nm范围，表面可带正或负电荷，且Zeta电位具有可调性。研究表明：牛血清蛋白（BSA）的包封率与起始的TMC、BSA浓度相关；纳米载药颗粒对BSA的释放表现为，先爆释而后缓释并可保持40h以上的释放。
N, N, N-trimethylchitosan chloride （TMC）, a water soluble derivative of chitosan, was synthesized by reaction between methyl iodide and chitosan. Based on contrarily electrostatic interaction process of TMC and carboxymethyl chitosan （CMC）, TMC/CMC nanoparticles with 200-600 nm in size were formed. The size, polydispersity, zeta potential and morphology of the nanoparticles were measured with dynamic laser scattering （DLS） and transmission electron microscopy （TEM）. Nanoparticles sizes were increased with the increasing concentration of TMC. Bovine serum albumin （BSA）, as a model protein drug, was incorporated into the TMC/CMC nanoparticles. The loading efficiency and release behave of the nanoparticles packed BSA were investigated. The loading efficiency was obviously decreased with the increasing of initial TMC and BSA concentration. In vitro release studies showed a burst effect and followed a slow and continuous release. The release could be kept for more than 40 h.
出处
《功能高分子学报》
CAS
CSCD
北大核心
2008年第1期50-54,75,共6页
Journal of Functional Polymers
基金
国家自然科学基金资助(30771419)
