摘要
以单分散亲水性交联聚甲基丙烯酸环氧丙酯-甲基丙烯酸乙二醇双酯(PGMA/EDMA)树脂为载体,制备新型L-羟脯氨酸聚合物键合高效手性配体交换固定相。该固定相在配体交换分离模式下,以0.2mol/LNaAc和0.1mmol/LCu(Ac)2水溶液(pH5.2)为流动相,柱温为30℃～50℃,对衍生和非衍生的D,L-氨基酸和α-羟基酸等9种手性化合物进行了高效液相色谱拆分。详细考察了流动相pH值、温度、流速和进样量对手性分离的影响,选择了合适的色谱分离条件。结果表明,所拆分的9种手性化合物,有5种手性化合物能得到基线分离,最好的分离因子α=2.32。
A new stationary phase was synthesized for chiral-ligand-exchange chromatography by treating the monodisperse poly （ glycidylmethacrylate-co-ethylenedimethacrylate ） particles with L-hydroxyproline. The enantiomeric resolutions of some D,L-amino acids and α-hydroxy acids were achieved on the synthesized chiral ligand exchange chromatographic stationary phase using 0.2 mol/L NaAc and 0.1 mmol/L Cu（Ac） 2 solution pH 5, 2 as mobile phase, 30℃ and 50℃ as the column temperature. The influence of the mobile phase pH,temperature of column, flow rate of eluent and sample concentration on the resolution of D,L-amino acids by ligand exchange chromatography were investigated. The results showed that five of the nine enantiomers could be separated from baseline and the best value of enantioselectivity（α） was 2.32.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2008年第3期275-279,共5页
Chinese Journal of Analytical Chemistry
基金
科技部国际科技合作重点项目(No.2006DFA3690)
教育部新世纪优秀人才支持计划(No.NCET-04-0986)
上海市博士后科研基金(No.06R214202)资助
关键词
手性配体交换色谱固定相
单分散树脂
L-羟脯氨酸
对映体分离
Chiral ligand exchange chromatographic stationary phase, monodisperse resin, L-hydroxyproline,enantiomeric separation
