摘要
目的探讨肝组织中 Fas 抗原的表达及其与病毒感染和细胞凋亡的关系。方法应用免疫组织化学方法检测了82例慢性肝病患者肝组织中 Fas 抗原、HBsAg 和 HCV NS5蛋白表达状况,并采用原位末端标记方法对部分患者肝组织中细胞凋亡状况进行研究。结果 64例检出 Fas 抗原(78.1%),检出率慢活肝和肝硬化组较慢迁肝组高,病毒抗原阳性组较阴性组高;抗原主要位于肝细胞浆,少数位于胞膜,阳性细胞主要分布在碎屑坏死周围,尤其在被炎性细胞分隔包围的肝细胞(岛)中表达显著;部分患者胆管上皮细胞亦可呈阳性;Fas 抗原阳性与阴性组间 ALT水平无显著差别,但阳性组组织活动指数(HAI)较阴性组高;对肝组织中凋亡细胞的原位检测发现,凋亡细胞多散布于肝实质中,在碎屑坏死及灶状坏死部位也可检出,但 Fas 抗原表达范围明显较凋亡细胞分布广泛;双染色研究发现,仅少数 Fas 抗原阳性细胞发生凋亡;凋亡细胞则绝大多数 Fas 抗原阳性.结论慢性肝病患者肝组织中 Fas 抗原表达与肝组织损伤程度有关并介导肝细胞凋亡。
Objective To inverstigate the expression of Fas antigen and its relationship with viral infection and apoptosis in liver tissue.Methods Fas antigen,hepatitis B surface antigen(HBsAg)and hepatitis C virus NS5 antigen(HCV NS5)were detected by immunohistochemical methodin 82 patients with chronic liver disease.Apoptosis were detected in some of these patients with in situ end-labeling method.Results Among the 82 subjects,64(78.1%) had been detected out Fas antigen in their liver;The detection rate was higher in patients with chronic active hepatitis and cirrhosis than that in patients with chronic persistance hepatitis;the same of that in patients with viral antigen(s) in their liver than those without viral antigens.The staining signal of Fas antigen was mainly located in the cytoplasm and less in the membrane of liver cells,most of the Fas antigen positive cells were distributed in areas with piecemeal or focal necrosis,especially in those separated and surrounded by inflammatory cells,a few patients showed Fas antigen in their bile duct epithelium.The ALT level had no difference between Fas antigen positive and negative group,but the histological active index(HAI)was higher in patients positive of Fas antigen than in those negative.Apoptosis cells,which visualized with in situ endlabeling,usually distributed scatered in liver parenchyma, but could also be detected in areas with piecemeal or local necrosis.The distribution of Fas antigen was much more wide than that of apoptosis.By the simultaneously staining of Fas and apoptosis,it was found that only a few Fas antigen positive cells showed apoptosis;on the other hand,most of the apoptosis cells were posistive for Fas antigen.Conclusion These results indicated that the expression of Fas antigen was related to liver injure degree, and it could mediated liver cell apoptosis.The significance of Fas antigen expression in liver tissue of patients with viral hepatitis and its influence factors need to be further study.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第1期19-21,共3页
Chinese Journal of Hepatology
