摘要
目的：探讨ＨＢＶ感染时的肝细胞凋亡及其参与的病理机制。方法：研究ＨＢＶ相关ＣＨ３８例、ＬＣ２７例和ＨＣＣ４３例的肝组织，以抗Ｆａｓ单抗检测Ｆａｓ表达及以标记的核苷酸借３＇未端转移酶检测ＤＮＡ损伤。结果：Ｆａｓ表达ＣＨ９４．７％、ＬＣ５９．３％、ＨＣＣ４１．９％，与炎症重度明显相关。ＤＮＡ损伤也与炎症一致。原位展现的凋亡细胞，主要在碎屑样坏死区。结论：ＣＴＬ－Ｆａｓ－凋亡也是参与肝细胞损伤的重要机制。在ＨＣＣ，Ｆａｓ还与突变型ｐ５３的表达相关，ｐ５３基因突变使细胞生长失控而癌变；细胞生长失控可能也是激发Ｆａｓ表达的信号。
Aim: The aim was to clarify the role between hepatocyte Fas expression and apoptosis in hepatitis B virus related chronic liver diseases. Methods: The liver tissues were investigated by in situ immunohistochemistry and TUNEL test. ResuIts: Fas expression in hepatcyte was found in 94.7% of 38 cases with chronic hepatitis. 59.3% of 27 with liver cirrhosis, and 41.9% of 43 with hepatocellular carci- noma respectively, significantly correlated with the severity of inflammatory lesions. The nuclei stained witl. TUNEL showed the 3'-OH end of DNA which marked impaired DNA, and also correlated positively with imflammation lesiocts. Apoptotic cells distributed mostly in the piecemeal necrotic region. ConcIusion: The CTL-Fas-apoptosis system was also involved in the mechanism of liver damage of hepatitis B. In hepatocellular carcinoma there was a relationship between the expression of Fas and p53 mutant.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第2期86-87,100,共3页
Chinese Journal of Hepatology
