摘要
目的了解庚型肝炎病毒(HGV)在慢性非甲-戊型肝炎发病中的作用,并测定其非结构基因5(NS5)区部分核苷酸序列。方法以逆转录-套式-聚合酶链反应法检测 HGV RNA,并对阳性者血清中扩增出的994bp 长的 cDNA直接测序。结果 35例慢性非甲-戊型肝炎患者中1例血清 HGV RNA 阳性(2.9%)。该患者 HGV NS_5区部分核苷酸序列与美国株 PNF2161及 R10291的同源性分别为87.95%及89.23%。与西非株的同源性为93.50%。结论 HGV 为慢性非甲-戊型肝炎的病原之一,但可能不是主要病原。在北京所分离的一株 HGVNS_5区核苷酸序列与西非株较接近。
Objective To elucidate the role of Hepatitis G virus(HGV)infection in chronic non-A-E hepatitis and analysis sequence the partial NS5 genome of HGV in the serum of patient with chronic non-A-E hepatitis.Methods Total nucleic acids were extracted from the sera of patients with chronic non-A-E hepatitis and subjected to reverse transcriptase-nested-polymerase chain reaction(RT-nested-PCR).Amplified cDNA,994 bp in length,was directly sequenced.Results HGV RNA was detected in 1 of 35 patients with chronic non-A-E hepatitis(HG-G). Compared with 2 American HGV isolates(PNF2161)and R1091),HGV NS5 gene of HG-G were 87.95% and 89.23% identical respectively,with West Africa HGV isolate(GBV-C),93.50% identical.Conclusions HGV is one of the causative agents of chronic non-A-E hepatitis,however,it may not be an important agent.The nucleotide sequence of partial NS5 gene of HG-G has high homology with GBV-C West Africa isolated.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第1期8-11,共4页
Chinese Journal of Hepatology
