摘要
将黄嘌呤氧化酶以共价连接法固定于二醋酸纤维素薄膜上，制成的酶膜与极谱式氧电极共同组成对次黄嘌呤进行定量测定的黄嘌呤氧化酶传感器。该传感器测定范围为１０～１２０ｍｇ／Ｌ，响应时间１０ｓ，不同浓度次黄嘌呤标准溶液对传感器１０ｓ响应值的相关系数为γ＝－０．９９６６，次黄嘌呤标准溶液重复测定６０次（１０ｓ响应值）的变异系数为ＣＶ＝０．３０３％。应用该传感器与分光光度法对鱼体次黄嘌呤含量进行了测定比较，二者具有良好的相关性。
Xanthine oxidase was immobilized on the cellulose acetate membrane by covalent binding. The enzyme membrane was attached on the top of an O 2 electrode to obtain a sensor specific to hypoxanthine. The assay range of the sensor was 10￣120 mg/L and CV=0.303%, r=-0.996 6, the response time was 10 seconds. The contents of hypoxanthine detected by the biosensor agreed well with those by the spectrophotometry.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第4期367-370,共4页
Chinese Journal of Veterinary Science
基金
军队"八五"重点课题
