摘要
采用逆相蒸发法制备稳定的白细胞介素2（IL－2）大单层脂质体，对其包封率、稳定性及活性进行了测定。建立C57BL／6小鼠荷瘤动物模型，通过给荷瘤小鼠腹腔注射空白脂质体、单纯IL-2及IL-2脂质体来比较其在肿瘤生长中的抑制作用，结果3组抑癌率分别为4．26％、34．04％和54.60％。空白脂质体组与单纯对照组相比尤显著性差异（P＞0．05），脂质体-IL-2组与单纯IL-2组之间存在显著性差异（P＜0．05），抑瘤率提高了20.6％。
Human recombinant interleukin-2 (IL-2) was entrapped in liposomes by reverse-phase evaporation method with an encapsulation efficiency of 34. 52±1. 12 %, and the antitumor effects of the liposomes containing IL-2 were studied using the C57BL/6 mice with Louis Lung carcinoma as an experimental tumor model. The iv injection of IL-2 liposome (5 333 U/g for the IL-2 dose) significantly inhibited the tumor growth as determined from the mean tumor weight (P<0. 05). Meanwhile, the untrapped IL-2 or liposome containing PBS was adndnistered to the mice in the same way. The inhibition rate of IL-2 liposome was increased by 20. 6 % compared with free IL-2.
出处
《中国生化药物杂志》
CAS
CSCD
1997年第4期166-169,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
白细胞介素2
脂质体
抑瘤率
Interleukin-2, Liposome, Inhibition rate of tumor
