摘要
目的探讨粉防己碱对人卵巢癌细胞株HO-8910增殖与凋亡的作用。方法MTT法测定细胞增殖情况;吖啶橙荧光染色(AO)观察细胞凋亡的形态学变化;流式细胞仪分析细胞周期及细胞凋亡。结果粉防己碱对HO-8910细胞有抑制增殖作用,呈时间、剂量依赖性;光镜可观察到细胞凋亡的形态学变化;流式细胞术检测结果分析,实验组G1/G0期上升,S期和G2/M期下降,细胞凋亡率上升。结论粉防己碱在体外能抑制卵巢癌HO-8910细胞增殖,诱导HO-8910细胞发生凋亡。
Objective To study the effect of tetrandrine on proliferation and apoptosis of ovarian cancer cell HO - 8910 in vitro. Methods The proliferation of cells was detected by MTT method. Morphological and ultrastructure changes were observed by AO staining. Cell cycle and apoptotic percentage were analyzed by flow Cytometry （FCM）. Results Tetrandrine could inhibit the proliferation of HO - 8910 cells directly related to concentration and time. Apoptosis morphology was observed in Tetrandrine groups. The flow cytometry showed that the proportion of G1/G0 phase and the apoptotic rate were increased significantly in Tetrandrine groups, the proportions of S phase and G2/M phase were reduced. Conclusions Tetrandrine can induce the apoptosis of ovarian cancer and inhibit cell proliferation.
出处
《辽宁医学院学报》
CAS
2007年第6期29-31,98,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
关键词
粉防己碱
卵巢癌
增殖
凋亡
tetrandrine
ovarian cancer
proliferation
apoptosis
