摘要
运用五种方法提取草珊瑚叶片总DNA。结果证明,加入PVP(聚乙烯吡咯烷酮)的改良CTAB法可以有效地提取产量高、质量高的草珊瑚叶片的总DNA。为草珊瑚及其它草珊瑚属植物的起源、分类、亲缘关系分析及品种特征图谱的建立提供可借鉴的依据。
The methods of CTAB procedure,SDS procedure and High salt procedure were used to find the best effective way for the genomic DNA isolation from Sarcandra Glabra (Thunb.) Nakai leaves in this paper. The result shows that CTAB procedure is the effective way for the genomic DNA isolation from Sarcandra Glabra (Thunb.) Nakai leaves.
出处
《新疆师范大学学报(自然科学版)》
2008年第1期87-89,共3页
Journal of Xinjiang Normal University(Natural Sciences Edition)
关键词
草珊瑚
总DNA提取
PVP
CTAB法
Sarcandra Glabra (Thunb.) Nakai
the Genomic DNA isolation
PVP
CTAB procedure