摘要
目的研究贯叶金丝桃中金丝桃素(HY)提取物对人乳腺癌细胞株MDA231的体外光动力杀伤效应。方法通过测定胞内荧光强度来确定细胞对金丝桃素的吸收,用显微镜观察、MTT法和DNA电泳分析法来研究金丝桃素对细胞的生长抑制作用。结果光活化的金丝桃素对MDA231细胞有显著的体外杀伤效应,其抑制细胞生长的能力与HY的浓度及光照能量密切相关。结论金丝桃素提取物在光照下能明显抑制乳腺癌细胞的生长,金丝桃素用于治疗乳腺癌具有广阔的开发应用前景。
OBJECTIVE To investigate the photodynamic therapy （PDT） antitumoral effect in vitro of Hypericin extract form Hypericum performatum L. on MDA231 human mammary carcinoma cell lines. METHODS The cellular uptake of Hypericin extract was investigated by detecting endocellular fluorescence, and the antitumoral effect of Hypericin extract was assessed by light microscopy, MTT assay and DNA ladder gel electrophoresis. RESULTS Light-activated Hypericin extract has an obvious inhibition effect against MDA231 cells in vitro, and its efficacy was correlated to the concentration of Hypericin extract and the light energy. CONCLUSION Hypericin extract can obviously decrease the growth of mammary carcinoma cells. Hypericin extract should be a powerful natural photosensitizer and have a good prospect for further investigation.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第1期1-4,共4页
Chinese Journal of Modern Applied Pharmacy
基金
深圳市科技局科技项目(03KJb019)
关键词
金丝桃素
金丝桃素提取物
光动力治疗
乳腺癌
Hypericin（HY）
Hypericin extract
photodynamic therapy（PDT）
mammary carcinoma
