摘要
复方中药注射液(ICHM)对人胃癌细胞 BGC-823和人乳癌细胞 MCF-7均有较强的杀伤作用。IC_(50)分别为0.02g 生药/mI 和0.018g生药/ml。流式细胞分析仪测定表明,ICHM 使 BDC-823细胞停止在 G_1期,导致 S 期细胞显著减少。通过~3H-TdR 掺入试验观察到,ICHM 能抑制 BGC-823细胞的 DNA 合成。
Previous studies had shown that injection of Chinese herbal mixture(ICHM)had antitumor and anti-metastatic actions on various transplantable animal tumors.In the present study,the authors observed the obvious killing actions of ICHM on human gastric cancer cell line BGC-823 and breast cancer cell line MCF-7 in vitro.The IC_(50)were 0.02 g crude drug/ml and 0.018 g crude drug/ml respectively.The study of the effect of ICHM on the cell-cycle of BGC-823 cell showed that ICHM decreased the number of cells in Sphase and increased the number of cells in G_1 phase significantly. The results indicated that the BGC-823 cells was blocked on S phase by ICHM.Meanwhile.ICHM reduced 3~H-TdR incorporation into DNA,the cpm value was much lower than that of the control.
出处
《中西医结合杂志》
CSCD
北大核心
1989年第7期416-417,390,共2页
