摘要
目的观察神经病理性疼痛(NPP)大鼠脊髓背角补体C3mRNA及蛋白的表达,探讨补体活化在NPP发病机制中的作用。方法84只健康雄性SD大鼠随机分为7组:正常对照组、假手术1、3、7天组和慢性坐骨神经压迫损伤(CCI)1、3、7天组(n=12)。CCI组大鼠左侧坐骨神经松结扎,假手术组暴露左侧坐骨神经,不做其他处理。分别于建模后1、3、7天测定大鼠热痛阈值和机械痛阈值,并通过RT-PCR、免疫比浊和免疫组化等方法观察大鼠脊髓背角C3mRNA及蛋白表达情况。结果CCI组大鼠坐骨神经结扎后1天即出现明显的机械和热痛过敏,结扎后3天和7天动物处在高水平的痛敏状态。假手术组大鼠未见明显的行为学变化。脊髓背角补体C3mRNA及蛋白的表达水平,CCI组大鼠在坐骨神经结扎后1、3、7天升高,且激活程度呈上升趋势,而假手术组与正常对照组间无明显差异。结论NPP大鼠脊髓背角补体C3mRNA及蛋白呈高表达状态,补体的这种变化可能在NPP的发生发展中发挥作用。
Objective To investigate the characteristic changes in the mRNA and protein of complement 3 in spinal dorsal horn of rats with neuropathic pain,and the role of abnormal activation of complement protein in the mechanism of pain production.Methods Eighty-four healthy male SD rats were divided randomly into seven groups（n=12 for each group）：normal control group,sham-operation 1d,3d and 7d groups,and chronic constriction injury of the sciatic nerve（CCI）1d,3d and 7d groups.The left sciatic nerve was ligated loosely in CCI groups,while it was only exposed but not ligated in rats in sham-operation groups.The mechanical and thermal pain thresholds were measured on 1,3 and 7 days after operation,and the mRNA and protein of complement 3 in the dosal horn of the spinal cord were determined respectively by RT-PCR,immunoturbidimetry and immunohistochemistry.Results The mechanical and thermal pain thresholds were observed to be lowered in rats one day after the sciatic nerve ligation,and a state of hyperalgesia was found to be persistent up to 7 days after CCI.This symptom was not observed in sham operation group.The expression of mRNA and protein of complement 3 in spinal dorsal horn were increased on 1,3 and 7 days after CCI.Interestingly,a high expression of mRNA of complement 3 was also observed in rats one day after sham-operation.Both mRNA and protein of complement 3 were not obviously elevated in rats of sham operation 3d,7d groups and normal control group.Conclusion The mRNA and protein of complement 3 in spinal dorsal horn are highly up-regulated in rats with neuropathic pain,suggesting that the characteristic dynamic changes in complement may contribute to the establishment and maintenance of hyperalgesia.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第12期1307-1309,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题(30772077)
关键词
坐骨神经痛
补体3
大鼠
sciatic nerve,complement 3,complement protein,neuropathic pain,rats
