摘要
在详尽分析α-酮戊二酸(α-KG)合成途径的基础上,结合数学模型,对光滑球拟酵母(Torulopsis glabrata)CCTCC M202019过量合成α-KG的合成途径及其调控因素进行全面分析与优化.全因子实验表明,硫胺素(B1)和Ca-CO3是影响α-KG过量积累的关键因素.在此基础上,采用最速上升实验得到α-酮戊二酸积累的最大响应区域为B10.016mg/L、CaCO384g/L附近.采用中心组合设计及响应面分析确定最优培养基组分为:硫胺素(B1)0.02mg/L、生物素(Bio)0.05mg/L、CaCO382g/L和乙酸钠4g/L.在最优培养基中,α-KG产量达到26.8g/L,提高了34%.α-酮戊二酸发酵过程动力学分析表明,采用最优培养基使发酵延滞期缩短6h,菌体比生长速率和α-KG比产物生成速率分别提高了45.4%和8.64%.
The concentrations of thiamine, biotin, CaCO3 and sodium acetate in fermentation medium for α-ketoglutaric acid （α-KG） production by Torulopsis glabrata CCTCC M202019 were optimized using the response surface method. Among these factors, thiamine and CaCO3 played key role in α-KG production based on the results from full factorial designing. Then, the ranges of tiliamine and CaCO3 were estimated by the steepest ascent. The optimum fermentation medium composition for α-KG production was determined by the central composite design as follows： 0. 02 mg/L thiamine, 0. 05 mg/L biotin, 82 g/L CaCO3 and 4 g/L sodium acetate. With the optimum conditions, the yield of α-KG reached 26. 8 g/L, and was 34% higher than that in the control （ original medium）. The kinetic analysis indicated that the lag phase was 6 h shortened, and the specific rate of growth and α-KG production were increased by 45.4% and 8.64% , respectively, compared with that of the control. Fig 2, Tab 3, Ref 12
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2008年第1期113-117,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金(No30670066)
国家杰出青年基金(No20625619)
长江学者和创新团队发展计划资助(NoIRT0532)
国家高技术研究发展计划(“863”计划,2006AA02Z01)项目资助~~
关键词
光滑球拟酵母
Α-酮戊二酸
响应面分析
动力学
Torulopsis glabrata
α-ketoglutaric acid
response surface method
kinetics
