摘要
Plant basic-leucine zipper （bZlP） transcription factors play important roles in many biological processes. In the present study, a bZlP gene, GmbZIP132, was cloned from soybean and its biological function under abiotic stresses was studied. The transcription of GmbZIP132 was induced by drought and high salt treatments. Among all of the organs analyzed, its expression was the highest in cotyUedon and stems. GmbZIP132 could weakly bind to the GCN4-1ike motif （GLM） （5＇-GTGAGTCAT-3＇） in yeast one-hybrid assay. Compared with wild-type （WT） Arabidopsis plants, transgenic plants overexpressing GmbZlP132 showed reduced abscisic acid sensitivity and increased water loss rate. At the stage of germination, transgenic plants were more tolerant to salt treatment than wild-type plants. The expression of some abiotic stress-related genes, such as rd29B, DREB2A, and PSCS, were upregulated in the transgenic plants. These results indicated that GmbZlP132 was an abioUc stress-related gene, and its overexpression could increase the salt tolerance of transgenic Arabidopsis plants during germination, yet no significant difference of tolerance to abiotic stresses was found between transgenic and wild type plants at the seedling stage.
Plant basic-leucine zipper （bZlP） transcription factors play important roles in many biological processes. In the present study, a bZlP gene, GmbZIP132, was cloned from soybean and its biological function under abiotic stresses was studied. The transcription of GmbZIP132 was induced by drought and high salt treatments. Among all of the organs analyzed, its expression was the highest in cotyUedon and stems. GmbZIP132 could weakly bind to the GCN4-1ike motif （GLM） （5＇-GTGAGTCAT-3＇） in yeast one-hybrid assay. Compared with wild-type （WT） Arabidopsis plants, transgenic plants overexpressing GmbZlP132 showed reduced abscisic acid sensitivity and increased water loss rate. At the stage of germination, transgenic plants were more tolerant to salt treatment than wild-type plants. The expression of some abiotic stress-related genes, such as rd29B, DREB2A, and PSCS, were upregulated in the transgenic plants. These results indicated that GmbZlP132 was an abioUc stress-related gene, and its overexpression could increase the salt tolerance of transgenic Arabidopsis plants during germination, yet no significant difference of tolerance to abiotic stresses was found between transgenic and wild type plants at the seedling stage.
基金
the National Natural Science Foundation of China (30490254)
the State Key Basic Research and Development Plan of China(2004CB117200).
