摘要
目的研究黄芪对幼兔肠缺血再灌注后肠黏膜细胞凋亡的影响及肠黏膜屏障的保护作用。方法雄性幼兔20只,分假手术组、肠缺血再灌注组(模型组)、黄芪组、生理盐水组(对照组);黄芪组在手术前7天予黄芪腹腔注射6mg/(kg·d),对照组每天予等量生理盐水。在手术时制作肠缺血再灌注模型,观察小肠组织及血浆超氧化物歧化酶(SOD)活性、肠黏膜细胞凋亡指数、肠黏膜形态损伤情况及黄芪对其改变的影响。结果肠缺血再灌注后血浆及小肠组织SOD活性明显降低,肠黏膜细胞凋亡指数明显增加,小肠黏膜形态及肠黏膜屏障功能损害加重,黄芪能显著改善上述改变。结论黄芪对幼兔肠缺血再灌注肠黏膜屏障损伤有明显的保护作用。
Objectives To study the protective effect of Redix astragail on intestinal mucosa barrier and intestinal mucosa apoptosis in infant rabbits with ischemia-reperfusion. Methods Twenty male infant rabbits were divided into 4 groups randomly： sham control group, model group, Redix astragail group and control group. Redix astragail group and control group were injected with Reclix astragail 6 mg/（kg·d） or sodium chloride 7 days before operation, respectively. SOD activity of intestinal mucosa and plasma, intestinal mucosa apoptosis index and degree of injury to intestinal mucosa tissue were observed in infant rabbits with ischemia-reperfusion. The influences of Redix astragail on them were also studied. Results After ischemia-reperfusion, SOD activity in intestinal mucosa and plasma decreased obviously, but intestinal mucosa apoptosis index, intestinal mucosa tissue morphology and intestinal mucosa barrier lesion increased. Redix Astragail could significantly improve the above changes. Conclusions Redix astragail played an important role in protection of intestine mucosa barrier of infant rabbits with ischemia-reperfusion.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2008年第2期142-144,共3页
Journal of Clinical Pediatrics
基金
贵州省合肥市科委资助项目(No.D-19)
关键词
肠缺血再灌注
凋亡
黄芪
小肠黏膜
intestinal ischemia-reperfusion
apoptosis
Redix astragail
intestinal mucosa
