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猪细小病毒NS1基因的克隆与序列分析 被引量：1

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus LJL12 Strain
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摘要 目的：扩增猪细小病毒（Porcine Parvovims，PPV）LJL12株NS1基因的全长序列，并进行同源性分析。方法：参考GenBank上公布的PPV中国株NS1基因序列，设计一对特异性引物扩增LJL12株NS1基因，测定序列，使用分子生物学软件进行同源性分析。结果：LJL12株PPV NS1基因全长1989bp，编码662个氨基酸。与其他PPV中国株NS1的核苷酸同源性在98．6％-100％之间，氨基酸同源性在98．5％-100％之间。其中，与南京株的同源性最高。结论：PPV NS1蛋白具有高度保守性，适合用作诊断抗原。LJL12株PPV NS1基因的克隆，为进一步研究NS1的功能和作用奠定了基础。 Objective：We determinate the sequences of NS1 Gene of Porcine Parvovirus LJL12 strain and analyze homologies of nucleotide sequences and amino acid sequences. Methods. According to the NS1 sequence of PPV Chinese strain in the GenBank, a pair of specific primers was designed to amplify the NSlgene of the LJL12 strain. Molecular biological soft wares were used to analyze homologies. Results： Integrated sequence of NSlgene has 1989 base pair, encodes 662 amino acid, exhibited 98.6% to 100% nucleotide sequence identity and 98.5% to 100% amino acid identity with other Chinese strains. The highest is the Nanjing strain among these. Conclusion： NS1 genc is highly conservative. It can be used in diagnosis. Molecular cloning of LJL12 NS1 genc makes it possible to disclose the function of NS1 protein.
作者 冉旭华 闻晓波 孟凡 周恩民
机构地区 黑龙江八一农垦大学动物科技学院
出处 《生物技术》 CAS CSCD 2008年第1期3-5,共3页 Biotechnology
关键词 猪细小病毒 NS1基因 序列分斩 porcine parvovirus NS1 genc sequence analysis
分类号 Q785 [生物学—分子生物学]
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参考文献9

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同被引文献15

  • 1卫功树.浅谈猪细小病毒与母猪繁殖失能[J].现代农业科技,2007(14):176-176. 被引量：7
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  • 6Cao S,Chen H,Zhao J,et al.Detection of porcine circovirus type 2,porcine parvovirus and porcine pseudorabies virus from pigs with post weaning multi systemic wasting syndrome by multiplex PCR[J].Vet Research Communications,2005,29 (3):263-269.
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  • 10Streck A F,Bonatto S L,Homeier T,et al.High rate of viral evolution in the capsid protein of porcine parvovirus[J].J Gen Virol,2011,92(11):2628-2636.

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