摘要
目的:建立不同来源知母药材 HPLC—ELSD 指纹图谱测定方法。方法:采用 HPLC—ELSD 法测定了18个来源不同的知母样品。色谱条件:Diamonsil^(TM)C_(18)(200 mm×4.6 mm,5μm)色谱柱,乙腑(A)-0.5%甲酸水溶液(B)为流动相,梯度洗脱[0～10 min,5%A→20%A;10～15 min,20%A→23%A;15～25 min,23%A;25～50 min,23%A→100%A;50～60 min,100%A],流速0.8 mL·mln^(-1)。柱温25℃。ELSD 检测器:漂移管温度110℃,雾化气体流速2.6 L·min^(-1)。结果:建立了不同来源知母的 HPLC—ELSD 指纹图谱,并进行相似度比较,对其中的知母皂苷 B-Ⅱ、知母皂甘 B-Ⅲ、anemarrhenasaponin Ⅰa、schidigera—saponin—F2、知母皂苷 A-Ⅲ进行了归属。其中前4个单体化合物为首次归属。结论:该方法操作简便、重复性好,可用于知母药材质量控制。
Objective： To establish the HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources. Method： The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources were obtained by Waters 600 instruments. The separation was performed on C18 analytical column（200 mm × 4. 6 mm ,5 μm）gradient eluted with a mixture consisting of acetonitrile（A） and 0. 5% formic acid solution（B） at a flow rate of 0. 8 mL · min ^-1 ,using a linear gradient of 5% A→20% A during 0 - 10 min;20% A→23% A during 10 - 15 min;23% A during 15 -25 min;23% A→100% A during 25 -50 min;100% A during 50 -60 min. The temperature of column was 25 ℃, The temperature of drift tube was 110 ℃, and the nebulizer nitrogen flow rate was 2. 6 L· min^-1. Result：The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae were established, and the similarity of medicinal materials from different sources was compared. Furthermore, four peaks in HPLC - ELSD chromatogram were firsdy assigned as timosaponin B -Ⅱ , timosaponin B - Ⅲ, anemarrhenasaponin Ⅰ a, schidigera - saponin - F2. Conclusion： The method is simple,reproducible and can be used as a quality control of Rhizoma Anemarrhenae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第1期100-103,共4页
Chinese Journal of Pharmaceutical Analysis
基金
中国科学院重要方向项目的部分研究内容(项目编号:KGUX2-SW-213-03)
