摘要
建立苦参碱白蛋白纳米粒药物包封率测定的 HPLC 法。方法:采用离心超滤法分离苦参碱白蛋白纳米粒中的游离苦参碱,以 HPLC 为分析手段对纳米粒包封率进行测定评价。色谱条件:采用 Kromasil C_(18)柱(4.6 mm×150 mm,5μm);流动相:乙腈-0.02mol·L^(-1)磷酸二氢钾溶液(6:94);流速:1.0 mL·min^(-1);检测波长:210 nm;温度:25℃。结果:采用离心超滤法分离,HPLC 法测定,可达到游离苦参碱与纳米粒的有效分离,苦参碱峰与溶剂峰分离良好,苦参碱浓度在6～100μg·mL^(-1)范围内线性关系良好(r=0.9999),平均回收率在95.06%～100.9%之间,日内 RSD 和日间 RSD 均小于2%(n=5)。结论:本方法准确可靠,简单快速,可用于苦参碱白蛋白纳米粒药物包封率的测定。
Objective： To establish an HPLC method for determination of entrapment efficiency of matrine - loaded albumin nanoparticles. Method： Free matrine （non -associated to the nanostructures） was separated from the nanoparticles by centrifugal ultrafihration technique, with HPLC for analysis for determination of entrapment efficiency of matrine - loaded albumin nanoparticles. Column ： Kromasil C18 （4. 6 mm × 150 mm,5 μm） ; Mobile phase ： acetonitrile-0. 02 mol· L^-1potassium dihydrogen phosphate solution（6： 94） at a flow rate of 1.0 mL· min^-1 ;UV detection wavelength was at 210 nm and temperature was 25 ℃;Results： Free matrine can be separated successfully from nanoparticles by centrifugal - uhrafihration technique, with HPLC for analysis for determination, the peak of matrine and solvent could be separated well. The calibration curve was linear in the range of 6 - 100 μg·mL^-1 with a correlation coefficient of r =0. 9999 ,the average recovery was 95.06% - 100. 9% ,the intra - day RSD and inter - day RSD were less than 2% （ n = 5 ）. Conclusion： This method is more accurate, reliable, simple and fast, and it can be used for determination of entrapment efficiency of matrine - loaded albumin nanoparticles.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第1期93-96,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
苦参碱白蛋白纳米粒
包封率
离心超滤法
HPLC
matrine -loaded albumin nanoparticles
entrapment efficiency
centrifugal ultrafiltration technique
HPLC
