摘要
以酶法合成γ-D-glutamyl-L-tryptophan(γ-D-Glu-L-Trp)为研究体系,采用D-谷氨酰胺为γ-谷氨酰基供体,L-色氨酸为受体,测定得到了转肽反应的米氏常数(Km)为5.11mmol.L-1,催化常数(Kcat)为3.92mmol.min-1,γ-D-Glu-L-Trp的水解反应米氏常数(K′m)为2.31mmol.L-1,催化常数(K′cat)为1.46mmol.min-1。采用顺序反应机制建立了酶法制备γ-D-Glu-L-Trp的过程动力学模型,并进行了参数优化。经验证,所建模型可准确预测该体系中的反应进程,模型数值解与实验值的平均相对误差小于5%。
A new model of enzymatic synthesis of γ-D-Glu-L-Trp （SCV-07） from D-glutamine and L- tryptophan was developed. The Km and Kcat of transpeptidation and enzymatic hydrolysis of product （presented as K＇m and K＇cat） were determined as Km =5.11 mmol · L^-1, Kcat =3.92 mmol· min^-1, K＇m= 2.31 mmol· L^-1 and K＇cat=1. 46 mmol · min^-1. The sequential mechanism was used to describe the rate of transpeptidation, and the model parameters were optimized. The enzymatic synthesis of γ-D-glutamyl-L- tryptophan was investigated to verify the model. Good agreement between experimental data and calculations was obtained. The total relative errors of experimental data and calculations were less than 5 %.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2008年第2期431-436,共6页
CIESC Journal
基金
江苏省高校"青蓝工程"项目
南京工业大学国家自然科学基金预研项目
