摘要
目的:鹿瓜多肽对去卵巢大鼠骨密度及松质骨中TGF-β1表达的影响。方法:将30只雌性SD大鼠,随机分为假手术(SHAM)组、去势(OVX)组、去势+鹿瓜多肽(OVX+CCP)组。术后20周测各组大鼠骨密度(BMD),利用免疫组织化学法观察各组大鼠松质骨中TGF-β1表达。结果:①OVX组与SHAM组相比较,松质骨中TGF-β1阳性细胞灰度值升高(P<0.05),CCP治疗组较OVX组灰度值降低(P<0.05);②与SHAM组相比,OVX组BMD明显降低(P<0.01);CCP治疗组各部位BMD高于去势组(P<0.05);结论:鹿瓜多肽注射液可以增加去势大鼠松质骨中TGF-β1的表达,抑制和延缓骨质疏松的形成,对雌激素缺乏引起的骨质疏松有预防作用。
Objective：To discuss the influence of cervus and cucumis polypeptide （CCP） on the expression of TGF - β1 in cancellated bone and bone mineral density （BMD） in ovariectomized rats. Methods：Thirty female SD rats were divided into three groups：sham operation （SHAM）group, ovariectomized （OVX）group and therapeutic （OVX ＋ CCP ）group. Bilateral ovariectomies were performed in OVX group and OVX ＋ CCP group. The ovariectomized rats in OVX ＋ CCP group were treated with CCP injection 1 day after operation. Twenty weeks after operation, the BMD of three groups was measured, and SABC immunohistochemistry was used to detect the expression of TGF - β1 in vertebrae bone. Results：The grayscale of cancellated bone in OVX group was significantly higher than that in SHAM group and OVX ＋ CCP group （ P 〈 0.05 ）. Compared with SHAM group, the BMD in OVX group was decreased significantly（P 〈0.01）, and the BMD in OVX ＋ CCP group was markedly higher than that in OVX group （P 〈 0.05 ）. Conclusion： The CCP could increase the expression of TGF - β1 in cancellated bone of ovariectomized rats and inhibit the occurrence of osteoporosis in ovariectomized rats.
出处
《西北国防医学杂志》
CAS
2008年第1期28-30,共3页
Medical Journal of National Defending Forces in Northwest China
关键词
骨质疏松
鹿瓜多肽
骨矿物质密度
转化生长因子-Β1
Osteoporosis
Cervus and cucumis polypeptide
Bone mineral density
Transforming growth factor - β1
