摘要
目的比较两种不同剂量的甲基强的松龙(MP)与单唾液酸神经节苷脂(GM-1)联合应用治疗大鼠实验性脊髓损伤的效果。方法36只大鼠随机分为3组,复制挤压性脊髓损伤动物模型,分别应用大剂量MP和两种不同剂量MP与GM-1联合治疗,术后24h、7d分别采血测定各组血清丙二醛(MDA)含量和后肢运动功能改良Tarlov评分。结果术后24h各组血清MDA含量显著升高,改良Tarlov评分显著降低,术后7d以上指标均有不同程度恢复,联合用药组较单独应用大剂量MP组恢复明显。其中,以小剂量MP+GM-1组恢复效果最明显。结论小剂量MP和GM-1联合应用治疗大鼠实验性脊髓损伤的效果优于另外两种药物疗法。
Objective To compare the effects of methylprednisolone （MP） of different doses combined with monosialotetrahexosyl ganglioside （GM-1） in treatment of spinal cord injury. Methods Thirty-six SD rats underwent compression of spinal cord so as to establish compressed spinal cord animal models, and then were randomly divided into three equal groups： high dose MP group （n=11）, undergoing injection of 30 mg/kg MP immediately after the operation, and then 5.4 mg/（kg.h） of MP in 4 dosages daily, all via caudal vein, moderate dose MP group （n=11）, undergoing injection of 15 mg/kg MP immediately after the operation, and 30 min later undergoing injection of GM-12 mg once daily for 5 d, all via caudal vein; and small dose MP＋ GM-1 group （n=12）, undergoing injection of 7.5 mg/kg MP immediately after operation, and 30 min later undergoing injection of GM-12 mg once a day for 5 d, all via caudal vein. 24 h and 7 d after the operation the movement of the hind legs were observed by double-blind method to record the modified Tarlov scores, and peripheral blood samples were collected from the caudal veins to detect the serum malonyldialdehyde （MDA）. Results Two rats, 1 in the high dose group and I in the moderate dose group, died after the operation. The Tarlov scores 24 h later of the high dose MP group, moderate MP＋GM-1 group, and low dose MP＋ GM-1 group were 2.32±1.14, 2.56±1.25, and 2.10±1.33, all remarkably decreased. The Tarlov scores 7 d later of the low dose MP＋GM-1 group and moderate MP＋GM-1 group were 5.88±1.15 and 4.93±2.05 respectively, both significantly higher than that of the high dose MP group （3.17±1.60, P 〈0.05 and P 〈0.01）, and the Tarlov score of the low dose MP＋GM-1 group was significantly higher than that of the moderate dose MP＋GM-1 group too （P 〈0.05）. The serum MDA levels 24 h after the injury of the 3 groups were all remarkably increased; and the serum MDA level 7 d later of the of the low dose MP＋GM-1 group and moderate MP＋GM-1 group were 3.15±1.58 and 4.44±1.09 nmol/ml respectively, both significantly lower than that of the high dose MP group （5.38±2.14 nmol/ml, both P 〈0.05 ）, and the serum MDA level 7 d later of the low dose MP＋GM-1 was significantly lower than that of the moderate dose MP＋GM-1 group （P〈0.05）. Conclusion The effect of low dose of MP combined with GM-1 in treatment of spinal cord injury is better than those of moderate dose of MP＋GM-1 and high dose MP.
出处
《中国急救复苏与灾害医学杂志》
2008年第2期89-91,共3页
China Journal of Emergency Resuscitation and Disaster Medicine
关键词
甲基强的松龙
神经节苷脂
脊髓损伤
Methylprednisolone
Monosialotetrahexosyl ganglioside （GM-1）
Spinal cord injury
