摘要
目的:分离肝癌细胞系MHCC97中边缘群细胞并观察其成瘤性和侵袭性.方法:利用流式细胞荧光激活分选法将肝癌细胞系MHCC97分成边缘群(SP)和主群(MP)细胞两个亚群.对两个亚群细胞分别采用软琼脂克隆形成实验和裸鼠成瘤试验观察其体内外成瘤能力;Transwell小室法检测两个亚群细胞的体外侵袭力.结果:SP细胞的体外克隆形成率为57%,MP细胞仅为13%;1×103个SP细胞可在4wk后形成明显的皮下移植瘤(2/8),MP细胞则需1×105个才能成瘤(2/8).Transwell侵袭试验结果显示,SP表型细胞穿过人工基底膜的细胞数为(66.6±4.0)个,MP表型细胞仅为(18.2±1.9)个.结论:肝癌细胞系MHCC97中SP亚群的成瘤性和侵袭性均强于MP亚群细胞,表明SP表型的细胞在肝细胞癌的生长、转移中具有重要的地位.
AIM： To isolate side population （SP） cells from MHCC97 cell line and to study their tumorigenicity and invasion. METHODS： Flow cytometry and Hoechst 33342 dye efflux assay were used to isolate SP cells and main population （MP） cells from MHCC97 cell line of human hepatocellular carcinoma. Then we observed the tumorigenicity of two subpopulations by soft agar cloning method and inoculation in nude mice. Invasiveness was measured by Transwell experiment. RESULTS： Fifty-seven percent of SP cells formed clones, while only thirteen percent of MP cells did. Tumor cell implantation experiment showed that only 1 × 10^5SP cells were sufficient for tumor formation （2/8）, whereas an injection of 1 × 10^5 MP cells could initiate tumors （2/8）. The cell number that infiltrated Transwell membrane in SP group （66.6 ± 4.0） was significantly higher than that in MP group （ 18.2 ± 1.9）. CONCLUSION： SP cells purified from MHCC97 cell line of human hepatocellular carcinoma show higher tumorigenicity and invading potential, indicating that SP cells have an important role in hepatooellular carcinoma cells proliferation and invasiveness.
出处
《第四军医大学学报》
北大核心
2008年第3期252-254,共3页
Journal of the Fourth Military Medical University
关键词
肝细胞癌
边缘群
肿瘤干细胞
侵袭
hepatocellular carcinoma
side population
stem cell
cancer stem cell
invasion
