摘要
为了分析除草剂的作用机理,用PSⅡ抑制型除草剂阿特拉津处理白菜幼苗,用2-DE技术和生物质谱方法分析叶绿体蛋白质组的变化,结果表明,23种叶绿体蛋白质斑点表现出明显且可重复的改变,其中4个下调,5个上调,14个表现出质的变化;在被处理植株中10个蛋白质斑点是新合成的;14个蛋白质斑点中的4个在高浓度(10mgL-1)Atrazine处理后消失了,但在低浓度(0.01、0.1和1mgL-1)Atrazine处理下没有变化。对14个发生了质变的蛋白质斑点进行了MALDI-TOFMS分析,其中7个分别被鉴定是糖基转移酶-Ⅰ、异戊二烯合成酶、一个经推测的蛋白质、脯氨酸脱氢酶、推测的6-果糖磷酸激酶异构酶Ⅱ、钙转运ATP酶和尿素酶K。同时也分析了阿特拉津处理对白菜幼苗叶绿素含量和可溶性蛋白质含量的影响,结果表明,经阿特拉津处理后叶绿素a含量下降,叶绿素b含量有上升趋势,但不显著。可溶性蛋白质含量随阿特拉津浓度的增加呈上升趋势。
To investigate the mechanism with which the herbicides works, chloroplast proteome was analyzed in Chinese cabbage seedlings treated with PS Ⅱ inhibiting herbicide Atrazine by using 2-DE techniques and MALDI-TOF MS. The results indicated that 23 protein spots showed significant and reproducible changes in treated-seedlings, of which 4 protein spots were down-regulated （spots 15-18） and 5 protein spots up-regulated （spots 19-23）. Fourteen protein spots showed qualitative changes between control and treated samples. Ten out of 14 proteins （spots 5-14） were newly synthesized in the treated seedlings. Four out of 14 protein spots （spots 1-4） disappeared treated with high concentration Atrazine （10 mg L^-1）, and not with low concentration Atrazine （0.01, 0.1, and 1 mg L^-1, respectively）. From the 14 protein spots, spots 1, 2, 5, 6, 8, 10, and 13 were respectively identified as glycos-transf-Ⅰ, isoprene synthase, hypothetic protein, proline dehydrogenase, putative 6-phospho-fructo kinase isozyme Ⅱ, calcium transporting ATPase, and maturase K, respectively. Changes in chlorophyll content and soluble protein content were also determined in treated seedlings. It was found that chlorophyll a content decreased, while chlorophyll b content showed a little increase without significant difference. The soluble protein content increased with increasing treatment concentration.
出处
《作物学报》
CAS
CSCD
北大核心
2008年第2期238-242,共5页
Acta Agronomica Sinica
基金
教育部科学技术研究重点项目(02010)
