摘要
目的分析布鲁杆菌毒力相关基因OMP25（编码外膜蛋白OMP25）在中国布鲁杆菌中的多态性分布，为研究致病机制，预防和控制大规模暴发布鲁菌病的流行病学研究提供理论基础。方法利用聚合酶链反应单链构象多态（single-strand conformation polymorphism，PCR-SSCP）方法检测116株布鲁杆菌（18株标准菌株和中国98株野生菌株）OMP25基因，取不同带型菌株的OMP25基因进行测序。结果116株布鲁杆菌呈现8种不同图谱（1～8型），其中2型和7型是个别中国野生株所特有。8种带型仅有10个不同碱基在对应类型中发生变异，对编码蛋白氨基酸的改变不多。中国98株布鲁杆菌的OMP25基因高度保守，具有比较稳定的抗原特征。结论高度保守性有助于布鲁杆菌的流行病学检测，为研制更安全有效的疫苗提供了理论依据。
Objective To investigate the genetic polymorphism of OMP25 gene isolated from 116 Brucella strains, including 18 Brucella reference strains and 98 Chinese field strains. Methods Chromosomal DNA of Brucella strains were analyzed by PCR, and then the product OMP25 gene was digested with Hind Ⅲ and separated on 10% polypropylene agarose gel electrophoresis. OMP25 genes of different types were sequenced. Results Eight gene types （ Ⅰ - Ⅶ） existing in 116 strains were examined, among them type Ⅱ and Ⅶ were only found in certain wild strains. Sequencing analysis of OMP25 revealed only 10 sites varied in all 8 types. OMP25 of 98 Chinese Brucella strains was highly homologic and characteristic of stable antigen. Conclusion High conservation characteristics helps to epidemiologically detect Brucella in China as well as to found a theoretical basis for vaccine development.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2008年第2期127-129,共3页
Chinese Journal of Preventive Medicine
基金
国家“973”科技攻关项目（2002CB513201）
关键词
布鲁杆菌属
细菌外膜蛋白质类
多态性
单核苷酸
聚合酶链反应
多态现象
单链构象
Brucella
Bacterial outer membrane proteins
Polymorphism, single nucleotide
Polymerase chain reaction
Polymorphism, single-stranded conformational
