摘要
目的对布鲁杆菌OMP28基因进行多态性分析,评价其作为基因标识的价值。方法根据聚合酶链反应-单链构象多态性(PCR-SSCP)分析技术原理,对中国120株布鲁杆菌的OMP28基因进行PCR扩增、酶切、电泳分析和筛选,取不同带型菌株的目的基因进行测序,将结果与Genbank中的布鲁杆菌OMP28序列进行比较。结果 120株布鲁杆菌呈现4种不同图谱(Ⅰ、Ⅱ、Ⅲ、Ⅳ),仅有3个碱基变异,即Ⅳ型与其他3个型第 304碱基A-G,Ⅱ型与另外3个型第405碱基C-T,Ⅰ与其他3个型第498碱基C-T变异,而且均未改变氨基酸序列。结论布鲁杆菌OMP28基因高度保守,具稳定的抗原特征。
Objective To evaluate the value of OMP28 being a gene marker through analyzing the gene type of OMP28 isolated from 120 strains of Brucella. Methods The sequence of OMP28 isolated from 120 strains of Brucella was analyzed using a PCR-SSCP strategy and the results were compared with that of Genbank. Results Four polymorphisms were identified （Ⅰ, Ⅱ, Ⅲ, Ⅳ） in all species examined. Mutations occurred only at 3 bases, which were A-G at the 304th base in type Ⅳ and C-T at the 405th base in typeⅡ and the 498th base in type Ⅰ. The sequence of amino acid kept unchanged. Conclusions OMP2g is highly homological and characterized by stable antigen.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第2期142-144,共3页
Chinese Jouranl of Endemiology
基金
国家"973"项目(2002CB513201)
关键词
布鲁杆菌
基因
多态现象
单链构象
Brucella
Genes
Polymorphism, single-stranded conformatiional
