摘要
目的 探讨西安市家畜戊型肝炎（戊肝）病毒（HEV）携带情况。方法收集猪、犬等常见家畜的胆汁标本，采用逆转录巢式聚合酶链反应（RT-nPCR）技术扩增HEV基因组开放读码2（ORF2）区域内150nt片段；阳性标本PCR产物直接测序，并与GenBank数据库进行比对，构建进化树分析遗传特征。结果共收集猪胆汁标本194份，犬胆汁标本178份，牛胆汁标本79份，羊胆汁标本191份。RT-nPCR检测结果表明，四种家畜胆汁标本HEVRNA阳性率分别为4．10％、0％、0％、0％；遗传距离分析发现本次得到的HEV与Ⅳ型HEV距离最近，其次为Ⅰ型、Ⅱ型和Ⅲ型；氨基酸水平也显示了同样的规律。结论猪是HEV家畜宿主，Ⅳ型是主要流行型。
Objective To explore the carrier state of hepatitis E virus（HEV） in livestock in Xi＇an area. Methods Bile samples from swine, canine, sheep and cow were collected from a local slaughtering house. Reverse transcriptase nested polymerase chain reaction （RT-nPCR） was employed to amplify the ORF2 region in HEV RNA genome. All positive samples were sequenced and compared with data from GenBank. Homology analysis was conducted based on the outcome of sequencing. Results 194,178, 79 and 191 bile samples from swine, canine, cow and sheep were collected. Positive rates with RT-nPCR method in these domestic animals were 4.10% , 0% , 0% and 0% respectively. Genetic distance analysis indicated that strains being identified were close to genotype Ⅳ of HEV, then genotype Ⅰ ,Ⅱ and Ⅲ in nucleic acid. Same outcome was shown by the same analysis on amino acid. Conclusion Swine was the only reservoir of HEV in livestock and genotype Ⅳ was the prevalent genotype.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2008年第2期158-160,共3页
Chinese Journal of Epidemiology
基金
国家自然科学基金资助项目（30771842）
