摘要
应用水提醇沉以及多种方法除蛋白,从五味子果实中获得一种粗多糖SCP-BII。SCP-BII经DEAE-Cellu-lose52和Sephadex G-200柱层析后得到单一组分SCP-BII。应用化学发光法对SCP-BII的清除·OH和O2·的能力进行了研究,结果表明:SCP-BII对·OH和O2·均具有明显的清除能力。
A polysaccharide SCP-B Ⅱ was obtained from Schisandra chinensis Turcz.Bail.fruits by method of water extraction and ethanol precipitation for protein removal. After DEAE-Cellulose 52 and Sephadex G-200 column chromatography, single component SCP-B Ⅱ was got. The scavenging ability of · OH and O2^- · of SCP-B Ⅱ were assayed by chemiluminescence. The results showed that SCP-B II have evident abilities of scavenging · OH and O2^-.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期91-94,共4页
Food Science
关键词
五味子多糖
分离纯化
清除自由基
Schisandra chinensis polysaccharides
purification
scavenging free radical
