摘要
利用荧光AFLP分子标记技术对26个枣优良品种及1个酸枣品种进行分子鉴别.8对引物共扩增得到886条带,其中特征性条带(某品种特有)112条,缺失条带(某品种缺少)60条,利用这些特殊性条带可有效地对供试材料进行鉴别.从3对引物中选择21条多态性丰富的DNA条带,将其转换成1、0数据,在此基础上开发出了用于枣优良品种鉴别的计算机应用软件.
Using fluorescent-labeled AFLP markers,this paper identifies 26 jujube cultivars and 1 sour jujube.Based on the number and quality of polymorphic fragments,8 AFLP primer pairs were selected for the identification.A total of 886 AFLP markers were identified,of which 112 makers were unique fragments and 60 markers were deletion fragments.These special markers have important use in jujube cultivars identification.Furthermore,21 fragments were selected from 3 primer pairs and changed to 1 and 0 data to establish a computer identification system.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2007年第6期119-121,共3页
Journal of Central South University of Forestry & Technology
基金
湖南省教育厅优秀青年项目(03B048)
湖南省自然科学基金(06JJ20022)项目
关键词
经济林
枣
品种鉴别
DNA指纹图谱
AFLP
计算机系统
non-timber forest
Ziziphus jujube Mill
cultivars identification
DNA markers
AFLP
computer system
