摘要
经特异免疫沉淀法和２．２，１５细胞株的ＨＢｓＡｇ、ＨＢｅＡｇ的抑制实验，以阿糖腺苷为阳性对照，从１０４种中草药中优选出单味中药（９１２）对ＨＢＶ－ＤＮＡＰ、ＨＢｓＡｇ和ＨＢｅＡｇ抑制率高达９０％左右。９１２经大孔吸附树脂粗分成１１个部位，其中Ｂ、Ｇ、Ｈ、１４个部位经抗ＨＢＶ及抗氧自由基实验证明为活性部位。经定性与定量分析，Ｂ、Ｇ、Ｈ、Ｉ中富含黄酮及多元酚类（鞣质）成分，鞯质含量与其抗ＨＢＶ活性成正相关。
We have tested 60 kinds of traditional Chinese herbs used in Clinical
treatment of liver diseases,by in vitro HBV-DNAP inhibition test,We
have also screened 44 kinds of medicines by the methodsof detecting
the HBsAg and HBeAg recreted from the 2,2,15 cell line derived from
the Hep-G2 celltransfected with cloned HBV-DNA. Ara-A was used as
positive control drug. The one most activeChinese traditional drug
found is“912”,its inhibition rates of HBV-DNAP、HBsAg and HBeAg are
allover 90%. By means of DA201 and H103 Macroreticular resins,11
fractions were separated from 912,Four active fractions B、G、H、I
were obtained by screening for Anti-HBV and Free-radical
inhibition.By quatitative and quantitative analysis,fractions( B、G、
H、 I)Found rich in flavonoids and
polyphe-nol(tannins)constituents,Their activity on anti HBV were
relatlve to their quantitative content of tannins.
出处
《北京医科大学学报》
CSCD
1994年第2期134-135,共2页
Journal of Peking University(Health Sciences)
关键词
中草药
多元酚
乙型肝炎病毒
Anti-Hepatitis B
Polyphenol
Chinese traditional drug
