摘要
目的探讨射频消融（RFA）治疗肝肿瘤大鼠后，其外周血、肝脏局部组织、脾脏组织内白细胞介素-10（IL—10）水平的变化及其意义。方法取30只Sprague-Dawley大鼠，制作肝肿瘤模型，随机分为RFA后1周组、RFA后2周组和对照组，每组10只。前2组分别于RFA处理后1周及2周处死，对照组不做RFA处理即处死。取射频灶周边（对照组取肿瘤周边）0．5cm范围内肝、脾组织及外周血，采用Ficoll密度梯度离心法分离出单个核细胞，应用流式细胞术检测IL-10表达水平。结果对照组大鼠肿瘤周边肝组织IL-10水平为（89．47±3.70），RFA后1周组为（81．62±10．19），RFA后2周组为（84．20±9．96），RFA后1周组与对照组比较差异有统计学意义；对照组大鼠脾脏组织IL-10水平为（96．32±0.89），RFA后1周组为（92．70±2.27），RFA后2周组为（96．34±0．97），RFA后1周组与对照组比较差异有统计学意义；对照组大鼠外周血IL-10水平为（95．92±2.31），RFA后1周组为（89．71±5．44），RFA后2周组为（87．67±11．11），RFA后1周组与对照组比较差异有统计学意义。结论RFA通过对肿瘤组织的原位灭活，在一定程度上解除肿瘤组织所释放的IL-10对荷瘤宿主的免疫抑制状态，削弱IL-10对机体抗肿瘤免疫应答，包括对机体及肿瘤局部树突状细胞分化成熟的抑制作用。
Objective To observe the change of IL-10 expression level in local liver and spleen tissues of rats with liver tumor treated by radiofrequency ablation （RFA）. Methods The experimental liver tumor model was established in 30 Sprague-Dawley rats by implantation of tumor particles. The model rats were then randomly divided equally into three groups： a lw after RFA group, a 2w after RFA group and a control group. The rats in the former two groups were treated with RFA once for 4 minutes, while those in the control group were not. The peripheral blood, spleen and liver tissues around the tumor of the rats in the control group, group 1 w after RFA and group 2w after RFA were sampled, respectively, before and at the time-points of 1 w and 2w after RFA. The mononuclear cells of peripheral blood,spleen and liver tissue were separated by Ficoll density gradient centrifugation. The expression level of IL-10 in peripheral blood, spleen and liver tissue were analyzed with flow cytometry. Results The expression level of IL-10 in local area around tumor of was 89.47 ± 3.7, 81.62 ± 10.19 and 84.2 ± 9.96, respectively, in the control group, group lw after RFA and group 2w after RFA. The difference between group lw after RFA and control group was significant. The expression level of IL-10 in spleen was 96.32 ±0.89, 92.7 ± 2.26 and 96.34 ±0.97, respectively, in the control group, group 1 w after RFA and group 2w after RFA. The difference between group 1 w after RFA and control group was significant. The expression level of IL-10 in peripheral blood was 95.92 ± 2.31 , 89.71 ± 5.44 and 87.67 ± 11. 11, respectively, in the control group, group lw after RFA and group 2w after RFA. The difference between group lw after RFA and control group was significant. Conclusion RFA can destroy the tumor tissues in situ and relieve immune suppression caused by IL-10 secreted from tumor tissue, decrease inhibitory action of these factors on antitumor immunity response to a certain extent. RFA can also improve differentiation and matura- tion of DC in local area of tumor and promote ability of antigen-presentation of the body.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第1期22-26,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
北京市科委重大项目培育专项（20005190040431）
关键词
射频消融
大鼠
肝肿瘤
白细胞介素-10
抗原提呈
Radiofrequency ablation
Rat
Liver tumor
lnterleukin-10
Antigen-presentation
