摘要
目的研究胸腔积液脱落细胞生存素基因表达及诊断价值。方法收集肺癌胸水脱落细胞阳性、肺癌胸水脱落细胞阴性及结核性胸膜炎患者胸水,提取其总RNA,采用逆转录-聚合酶链反应(RT-PCR)检测生存素基因mRNA表达。结果42例肺癌患者胸水脱落细胞生存素mRNA阳性率80.95%(34/42),胸水脱落细胞阴性者阳性率52.38%(20/42),30例结核性胸膜炎均为阴性。结论胸水脱落细胞生存素mRNA可以作为肺癌诊断新的敏感指标。
Objective To study the clinical value of survivin gene expression in hydrothorax exfoliative cells in the diagnosis of lung cancer. Methods Positive and negative exfoliative cells were collected from the hydrothorax in the patients with lung cancer or tuberculosis pleurisy. The survivin gene expression in the cells was detected by RT-PCR. Results The positive rate of survivin mRNA was 80. 95M （34/42） in 42 lung cancer patients with positive exfoliative cells, and 52.38% （20/42） with negative exfoliative cells. There was no survivin mRNA expression in hydrothorax from tuberculosis pleurisy patients. Conclusion The expression of survivin mRNA in hydrothorax exfoliative cells may be used as a new sensitive marker for diaznosing lung cancer.
出处
《江苏医药》
CAS
CSCD
北大核心
2008年第1期47-49,共3页
Jiangsu Medical Journal
关键词
生成素基因
胸腔积液
肺癌
Survivin mRNA
Hydrothorax
Lung cancer
