摘要
目的探讨DNA倍体分析在良、恶性胸腔积液诊断中的临床价值。方法应用流式细胞术（FCM）检测24例良性胸腔积液和39例恶性胸腔积液中细胞核DNA倍体并进行分析，并同时与细胞学检测结果进行比较。结果良、恶性胸腔积液中，FCM检测的阳性率分别为8．33％（2／24）、64．10％（25，39），差异有统计学意义（P〈0．05）；细胞学检测的阳性率分别为4．17％（1／24）、53．85％（21／39），差异有统计学意义（P〈0．05）。在恶性胸腔积液中，FCM、细胞学检测的敏感度分别为64．10％（25／39）、53．85％（21／39），特异度分别为91．67％（22／24）、95．83％（23／24），两种方法比较差异均无统计学意义（P〉0．05）。结论流式细胞术DNA倍体分析对于良、恶性胸腔积液具有重要的临床诊断意义。
Objective To investigate the chnical value of DNA ploidy analysis in the diagnosis of benign and malignant pleural effusion. Methods DNA ploidy in 24 benign pleural effusion and 39 malignant pleural effusion were detected by flow cytometry （FCM） and compared with the results of cytologic detection at the same time. Results The positive rates of FCM detection in benign and malignant pleural effusion were 8.35%（2/24） and 64.10%（25/39）,there was significant difference （P 〈 0.05）. The positive rates of cytologic detection in benign and malignant pleural effusion were 4.17% （1/24） and 53.85%（21/39）, there was significant difference （P 〈 0.05）. The sensitivity of FCM and cytologic detection in malignant pleural effusion was 64.10% （25/39） and 53.85% （21/39）,the specificity of two methods was 91.67% （22/24） and 95.83% （23/24）,the results of two methods showed no significant differences （P 〉0.05）. Conclusion DNA ploidy analysis by FCM has important clinical value in the diagnosis of benign and malignant pleural effusion.
出处
《中国医师进修杂志》
2012年第16期28-30,共3页
Chinese Journal of Postgraduates of Medicine
