摘要
目的了解南京地区淋球菌青霉素耐药状况及产青霉素酶淋球菌的质粒基因型的流行情况。方法采用琼脂稀释法检测淋球菌对青霉素的最小抑菌浓度采用纸片酸度定量法检测菌株是否产青霉素酶。采用单管PCR方法对产青霉素酶淋球菌耐药质粒进行基因分型。结果8年间共检测1208株淋球菌，青霉素总耐药率为84．02％（1015／1208）。非产青霉素酶淋球菌中，染色体介导的耐青霉素淋球菌占74．02％（550/743）。产青霉素酶淋球菌占38．49％（465／1208），其中177（38．06％）株同时为质粒介导的耐四环素淋球菌即质粒介导的高度耐四环素淋球菌。产青霉素酶淋球菌阳性率自1999（8．04％）年起逐年增加，至2004年达最高（57．36％），2005、2006年略有下降。质粒PCR分型显示，所测菌株均携带亚洲型质粒。结论南京地区耐青霉素淋球菌始终维持在较高水平，产青霉素酶淋球菌近年增加较快，携带亚洲型质粒的产青霉素酶淋球菌在南京地区流行，未发现其他类型的质粒。
Objective To study the penicillin resistance in Neissena gonorrhoeae isolates and gcnotypes ofplasmid in pcnicillinasc-producing N. gonorrhoeae isolates （PPNG） from Nanjing. Methods The minimum inhibitory concentrations （MIC） of penicillin were determined by agar dilution method. The production of pcnicillinasc was examined by paper acidomctric testing. The gcnotyping of resistance plasmid in PPNG was carried out by a single tube PCR. Results During the past eight years, 1208 N. gonorrhoeae isolates were examined. Among all the isolates, 84.02% （1015/1208） were resistant to penicillin, 38.49% （465/1208） were pcnicillinasc-producing, and 38.06%（ 177/465 ） of the PPNG were resistant to tetracycline mediated by plasmids. The penicillin resistance was mediated by chromosomes in 74.02% （550/743） of non-PPNG. The proportion of PPNG had increased year by year, flom 8.04% in 1999 to 57.36% in 2004, and decreased slightly in 2005 and 2006. The results of gcnotyping showed that all the tested PPNG carried the Asian type plasmid. Conclusions The prevalence of penicillin-resistant N. gonorrhoeae maintains at a higher level in Nanjing, and the proportion of PPNG has increased rapidly in recent years. Asian type is the predominant type of plasmid in PPNG, none of other types of plasmid was found in this study.
出处
《国际皮肤性病学杂志》
2008年第1期4-6,共3页
International Journal of Dermatology and Venereology
