摘要
为研究中医“恐伤肾”、“肾为先天之本”等理论，设计了以家猫惊恐孕鼠再用四甲基偶氮唑盐（ＭＴＴ）改良法检测子代鼠自然杀伤细胞活性（ＮＫＣＡ）的实验。结果表明：惊恐组子代鼠ＮＫＣＡ明显高于对照组（Ｐ＜０．０１）；补肾组、反证组与对照组子代鼠ＮＫＣＡ间比较无统计学意义（Ｐ＞０．０５）。提示孕鼠在惊恐应激后神经内分泌免疫内环境的改变可能影响到其子代鼠的先天之本，导致其“肾气”发生适应性代偿性的功能异常增强反应。而经典补肾方药金匮肾气丸对此有一定调节作用，但对正常孕鼠子代鼠ＮＫＣＡ的影响不显著。
In order to research the basic theory of 'TCM as fright impairs kidney' and 'the kidney being the origin of congenital constitution,' experiment was designed as frightening pregnant mice with cat and measuring the natural killer cell activity (NKCA) of its filial generation by improved MTT colorimetric assay. The results Showed that the NKCA of fright group was significantly higher than that of control group(P<0.01) and no significant difference lied among the kidney-tonified group,counter group and control group(P>0.05).It was suggested that the change of neuroendocrinoimmunological internal enviroment after the pregnant mice being fright-stressed might affect the congenital constitution of the filial generation, causing adaptive cocompensatory abnormal enhancement of its kidney-qi, modulated by Gold-chamber Kidney-qi pill ,which had no significant influence on the NKCA of filial mice from the normal fright-free pregnant mice.
出处
《成都中医药大学学报》
1997年第2期33-34,共2页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金
关键词
肾
惊恐
动物实验
NKCA
NK细胞
Kidney(TCM)
Fright(seven emotion) stress
Natural killer cell activity
