摘要
目的探讨利多卡因联合地塞米松局部封闭治疗20%甘露醇外渗4h后局部损伤的效果。方法将7只小白鼠四肢内侧制成20%甘露醇外渗模型。4h后,随机抽取1只小白鼠于四肢注射部位取材(E组),观察甘露醇外渗致局部组织损伤情况;其余6只按模型部位分为A组(左上肢)、B组(左下肢)、C组(右上肢)及D组(右下肢)。A组予2%利多卡因2ml加地塞米松1mg局部封闭,B组予2%利多卡因2ml局部封闭,C组予生理盐水局部封闭,D组为空白对照。处理24h后取材进行病理学观察。结果光学显微镜观察显示,E组局部组织损伤呈重度表现;A组呈无或轻度表现,轻于B组、C组、D组和E组;B组以中度为主,轻于C组、D组(均呈重度)和E组。结论甘露醇外渗后4h组织损伤严重,外渗后需及时采取有效措施,避免组织损伤加重;利多卡因联合地塞米松封闭治疗甘露醇外渗4h形成的局部组织损伤快速、简便并安全有效。
Objective To observe the therapeutic effects of lidocaine combined with dexamethasone acetate injection on mannitol extravasation-induced injury for 4 h. Methods Seven mice were included in this experiment. Mannitol extravasationqnduced skin injury models were established. After 4 h,one mouse （group E. four limbs） wasn＇t treated, the left 6 mice were treated with lidocaine combined with dexamethasone acetate （group A, upper-left limb）, lidocaine （group B, lower-left limb）, normal saline （group C. upper-right limb）, and group D （lower-right limb） without any treatment. Effects were assessed by pathological detection after 24 h. Results The local tissues in group C, group D and group E showed severe injuries, however, less or milder injuries in group A and moderate injures in group B were detected after injection block therapy. Conclusion Mannitol extravasation for 4 h can induce severe injuries, so measures must be taken quickly. Lidocaine combined with dexamethasone acetate injection block therapy to treat injuries caused by mannitol extravasation for 4 h is safe, quick, convenient and effective.
基金
2004年南京医科大学科技发展基金资助项目(NY04031)
关键词
甘露醇
药物外渗
疾病模型
利多卡因
地塞米松
动物实验
mannitol
drug extravasation
disease model
lidocaine
dexamethasone acetate
animal experiment
