摘要
目的:探讨应用兔骨髓基质细胞与生物活性玻璃复合培养构建组织工程化人工骨的可行性。方法:将体外分离培养的兔骨髓基质细胞,经向成骨方向诱导分化后,与生物活性玻璃进行联合立体培养,通过扫描电镜观察骨髓基质细胞在生物活性玻璃上的生长情况。结果:骨髓基质细胞可在体外增殖和向成骨方向分化,并且可在生物活性玻璃上黏附和铺展。结论:骨髓基质细胞与生物活性玻璃复合培养可构建具有活细胞成分的组织工程化人工骨。
Objective:To approach the feasibility of rabbit marrow stroma cells combined with biological activity glass frame to construct tissue-engineering artificial bone. Methods: Rabbit marrow stroma cells, isolatedly cultured in vitro and differentiated inducedly to direction of bone formation, were combined with biological activity glass frame for three-dimensional cultivation. Informations of rabbit marrow stroma cells among biological activity glass frame were observed by scanning electron microscope. Results: It showed that marrow stroma cells could not only generate in vitro and differentiate to direction of bone formation, but also paste and extend on biological activity glass frame. Conclusion: It indicated that compound culture of marrow stroma cells and biological activity glass frame could construct dssue-engineering artificial bone with living cell constituents .
出处
《中医正骨》
2008年第1期9-10,共2页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
山东省科技厅资助项目(2002年度山东省中医药科技发展计划第13号)
关键词
骨髓基质细胞
生物活性玻璃
复合培养
组织工程化人工骨
实验研究
动物
免
Marrow stroma cell, biological activity glass, compound cultivate, tissue-engineering artificial bone, empirical study, animal, rabbit
