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正交试验优选珠芽蓼果实中总黄酮提取工艺 被引量:6

Optimization of Extract Technology of Total Favonoids in Fruit of Polygonum viviparum L.by Orthogonal Experiment
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摘要 目的:优选珠芽蓼果实中总黄酮的提取工艺。方法:以芦丁为标准品计算总黄酮含量,通过单因素试验和正交试验考察不同因素和水平对提取工艺的影响。结果:最佳提取工艺为80℃水浴温度,液固比为8∶1,乙醇浓度为50%,回流提取2次,每次1h。在此条件下,总黄酮提取率为9.6%。结论:该提取工艺合理、简单、可行,可为大生产提供理论依据。 OBJECTIVE: To optimize the extract technology of the total Favonoids from the fruits of Polygonum viviparum L.METHODS: The content of Flavonoids was computed with rutin as standard substance, and the influence of different factors and levels on the extract technology were investigated by means of simple factor experiment and orthogonal experiment. RESULTS: The optimum extraction technology was as follows: with water- bath temperature at 80℃, the ratio of liquid to solid material at 8 : 1 and the concentration of ethanol at 50%, reflux extracting total Favonoids twice(1 h at each time) . Under this condition, the content of total Flavonoids was 9.6% . CONCLUSION: The extraction method is appropriate, simple and feasible, and it provided theoretic basis for the production of this product.
出处 《中国药房》 CAS CSCD 北大核心 2008年第3期186-188,共3页 China Pharmacy
关键词 珠芽蓼 果实 总黄酮 提取工艺 正交试验 Polygonum viviparum L. Fruits Total Favonoids Extract technology Orthogonal experiment
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