摘要
目的研究溃疡性结肠炎(UC)患者外周血CD4+CD25+调节性T细胞数量、相关细胞因子的水平以及Foxp3基因的表达,分析它们在UC发病机制中的作用。方法收集UC患者及健康对照组外周抗凝静脉血,分离纯化T淋巴细胞。PE标记的抗CD4单抗,FITC标记的抗CD25单抗,作双色流式细胞术,分析UC患者外周血CD4+CD25+调节性T细胞百分率,ELISA法检测血清中相关细胞因子IL-10、TGF-β1的水平,RT-PCR检测T细胞Foxp3 mRNA的表达。结果与正常对照组相比,UC患者外周血CD4+T、CD25+T细胞百分率作及CD4+CD25+T/CD4+T均显著下降(P均<0.05),细胞因子IL-10、TGF-β1的水平也显著降低(P<0.05),T细胞Foxp3 mRNA的表达也明显降低(P<0.05)。结论UC患者外周血存在细胞免疫功能失调,CD4+CD25+调节性T细胞数量与功能状态发生改变,T细胞耐受机制的破坏可能与UC的疾病活动状态和发病机制有关。
Objective To investigate the quantity of peripheral blood CD4^＋ CD25^＋ regulatory T cell, related cytokines and the expression of Foxp3 gene in ulcerative colitis（UC） patients, and to analyze the role of them in the pathogenesis of UC. Methods Anti-coagulated venous blood by heparin was collected from UC patients and healthy people. T cells were collected by Human CD3^＋ T cell enrichment column. The quantity of peripheral blood CD4^＋ CD25^＋ regulatory T cell was detected by immunofluorescence staining and bicolor flow cytometry by CD25/CD4 gating. The levels of related cytokines IL-10,TGF-β1 were assayed by ELISA, the expressions of Foxp3 mRNA on T cell were analyzed by RT-PCR. Results Compared with healthy people, in UC patients, the expression of CD4^＋ CD25^＋T cell and the level of Foxp3 mRNA both decrease significantly （P 〈 0.05 ）. The levels of related cytokines IL-10,TGF-β1 were lower than those of control group. Conclusion There is peripheral blood celluar immunological function disorder in UC patients, and the abnormal expression of CD4^＋ CD25^＋ T cell , the related cytokines and Foxp3 mRNA on T cell may involve in UC immunopathogenesis.
出处
《咸宁学院学报（医学版）》
2007年第6期483-486,共4页
Journal of Xianning Univarsity(medical Sciences)
