摘要
[目的]了解四川地区引起尖锐湿疣的HPV-6和11亚型早期基因E6、E7的结构和变异特点。[方法]采用PCR和T-A克隆技术从尖锐湿疣样本中扩增、克隆HPV-6/11E6、E7基因,最后对其测序并与原型进行序列比对。[结果]成功扩增和克隆了HPV-6/11E6、E7基因,其序列与原型序列相比,存在多处核苷酸的突变,并且有几处核苷酸的突变引发了氨基酸序列的突变,其中有3处引起氨基酸的突变为所有样本共有:HPV-6E6:252G→C(谷氨酰胺→组氨酸);HPV-6E7:685A→T(酪氨酸→苯丙氨酸);HPV11E7:662G→T(丙氨酸→丝氨酸)。[结论]四川地区HPV-6和11亚型E6、E7基因均存在一定程度上的变异,大部分的突变为同义突变,E6基因较E7基因更易突变。
[Objective ] To investigate structure and mutant characteristics of E6 and E7 genes of HPV-6 and 1 lsubtype in condyloma acuminatum in Si Chuan province. [ Methods Z Samples of condyloma acuminatum were amplified by PCR and T- A cloning technique, and E6 and E7 genes of HPV-6 and 1 lsubtype were cloned, finaUy all the genes were sequencd as well as sequence of these genes were compared with prototype genes. [ Results] The E6 and E7 genes of HPV-6 and llsubtype were successful amplified and cloned. When compared with the sequence of prototype genes, several nucleotide mutations were found in E6/E7 genes, and some mutations had induced the change of amino acid sequence, three point mutations of amino acids were found in all the condyloma acuminatum samples： HPV-6E6： 252G→C （glutamine→histidine） ; HPV-6E7： 685A→T （tyrosine→phenylalanine） ; HPV-11E7：662 G→T （alanine→serine）. [Conclusion] In Si Chuan province, both E6 and E7 genes of HPV-6 and llsubtype can mutate to some extence, and most mutations were samesense mutation, while E6 gene mutates easier than E7 gene.
出处
《现代预防医学》
CAS
北大核心
2008年第2期316-318,共3页
Modern Preventive Medicine
关键词
尖锐湿疣
人乳头瘤病毒
基因克隆
序列分析
Condylomata acuminate： Human papUlomavirus
Gene coloning
Sequence analysis
