摘要
采用正相和反相高效液相色谱法对硝虫硫磷进行定性分析,并确定用反相高效液相色谱法对硝虫硫磷进行定量分析.反相高效液相色谱条件:使用C18反相柱和二极管阵列检测器,以乙腈+水=65+35(V/V)为流动相,柱温30℃,流速:1.2 mL/min;,检测波长220 nm,外标法对硝虫硫磷的有效成分进行定量分析.结果表明硝虫硫磷的线性关系系数为0.999 6,测定30%硝虫硫磷乳油时,标准偏差为0.19,变异系数为0.62%,平均回收率为99.84%;测定硝虫硫磷原药时,标准偏差为0.08,变异系数为0.09%,平均回收率为100.16%.
An HPLC method was used to qualitatively and quantitatively analyze the active ingredient of xiaochongthion including PP-HPLC method and RP-HPLC method. RP-HPLC method was used to quanlititively analyze xiaochongthion. The condition of RP-HPLC method： The mobile phase was Acetonitrile ＋ water（65 ＋ 35, by vol）. Column temperature was 30 ℃, flow rate was 1.0 mL/min, and detection wavelength was 220 nm. The results showed that the linear correlation coefficient was 0. 999 6, the standard deviation was 0. 19 ,the variation coefficient was 0.62%, the average recovery was 99.84% when 30% Xiaochongthion EC was detected; the standard deviation was 0.08, the variation coefficient was 0.09%, the average recovery was 100. 16% when Xiaochongthion TC was detected.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期121-125,共5页
Journal of Southwest China Normal University(Natural Science Edition)
关键词
硝虫硫磷
高效液相色谱
分析
xiaochongthion
high performance liquid chromatograph
analysis
