摘要
采用Ames试验和小鼠淋巴瘤致突变试验(MLA)来检测单方马兜铃及复方龙胆泻肝丸的遗传毒性,评价其相关细胞毒性和复方减毒效果;为进一步建立综合的中药遗传毒性测试平台提供试验依据.分别通过对含马兜铃酸(Aristolochic Acid,AA)浓度为20和40μg/mL,加S9或不加S9的条件下的两味中药的Ames法检测;以及采用MLA96孔微孔板接种法分别对单复方含马兜铃酸浓度为5μg/mL的L5178Y/tk+/--3.7.2c细胞进行染毒,并进行接种效率(PE),相对总增长率(RTG)和突变频率(MF)的测定.结果表明单方马兜铃具有细胞毒性且致突变性较强,以诱导大范围DNA损伤为主;而复方龙胆泻肝丸具有较明显的减毒效果;MLA和Ames试验组合适用于体外中药遗传毒理检测.
To detect the different mutagenicities, cytotoxicities of Fructus Aristolochiae （FA） and Long-Dan-Xie-Gan-Wan （LDXGW）, single and compound prescriptions in Chinese Traditional Medicine （CTM） respectively, via the means of Ames Test and Mouse Lymphoma Assay （MLA）. The mutation frequency caused by FA and LDXGW, which contained Aristolochic Acid （AA） with the concentration of 20μg/mL and 40μg/mL in two tests,were detected by conducting the normal Ames tests, with or without S9 added. MLA： L5178Y tk ^＋/- - 3.7.2c cells were treated with FA and LDXGW, with the concentration of AA as 5μg/mL. The plating efficiency （PE） ,relative total growth （RTG） and the mutant frequency （MF） were detected by the microtiter procedure. FA is characterized by marked cytotoxicity and mutagenic activity. Its mutagenicity may result in large range of DNA damage; while LDXGW shows that CTM＇s cytotoxicity and mutagenic activity might be largely reduced when made into a compound. MLA is much more sensitive than Ames Test in genetic toxicity test. The virtue of the combination of two methods was explored at the first trail.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2007年第6期992-995,1000,共5页
Journal of Fudan University：Natural Science
基金
国家自然科学基金资助项目(30472110)
