摘要
为探讨单磷酰脂Ａ（ＭＬＡ）的心脏保护作用与诱导金属硫蛋白（ＭＴ）产生之间的关系，本实验在大鼠离体灌注心脏缺血再灌注（Ｉ／Ｒ）模型上观察ＭＬＡ预处理２４ｈ后，对心脏缺血再灌注损伤及ＭＴ含量的影响。结果发现，ＭＬＡ预处理增加心肌ＭＴ含量达４．６倍（ｐ＜０．０１），并增加了对Ｉ／Ｒ的抵抗，表现为改善了心功能和增加了心肌能量储备（ＡＴＰ含量升高）及抑制心肌酶和肌红蛋白的漏出。应用ＭＴ抗血清消除了ＭＬＡ预处理的心脏保护作用。结果表明，诱导ＭＴ产生可能是ＭＬＡ预处理心肌保护作用机理之一。
To investigate the relationship between the cardioprotective effect of monophosphoryl lipid A(MLA) and production of metallothionein (MT), the present study observed the effect of MLA precoditioning on ischemia/ reperfusion (I/R) injury of perfused rat heart and myocardial MT content. It was found that MLA preconditioning increased myocardial MT content to 4.6fold (p<0.01) and potentiated the resistance to I/R injury, i.e.improved cardiac function, increased myocardial energy reservation (ATP content), and lowered the leakage of tissue LDH and myolobin, Adding MT antiserum to perfusate abolished the cardioprotective effects of MLA preconditioning. The results indicated that lnduction of MT production might be one of the cardioprotective mechanisms of MLA preconditioning.
出处
《基础医学与临床》
CSCD
1998年第1期56-59,共4页
Basic and Clinical Medicine
关键词
心脏缺血
再灌注损伤
心脏保护
金属硫蛋白
MLA
preconditioning\ \ heart\ \ ischemia/reperfusion injury\ \ Metallothionein\ \ monophosphryl Lipid A
