摘要
将１３日龄的石歧杂公鸡分成６组，第１组于７８日龄进行人工阉割，第２、３、４、５组分别以促性腺激素（ＬＨＲＨ）－ＢＳＡ偶联物作抗原加佛氏佐剂乳化后按不同程序免疫公鸡，第６组单以ＢＳＡ免疫作对照。结果表明：与ＢＳＡ免疫组相比，第４、５组及阉鸡组血清睾酮水平均发生显著下降，各免疫去势组公鸡睾丸、鸡冠及肉垂都有不同程度的萎缩，睾丸曲细精管发育不良或受到抑制，６０日龄免疫去势效果明显比２０及４０日龄免疫的差，以相同的抗原量两次免疫去势效果优于一次免疫效果，其中以ＬＨＲＨ－ＢＳＡ加佛氏佐剂分别于２０及４０日龄各免疫一次可获得较好的去势效果。
Thirteen day old Shiqi cockerels were divided into 6 groups, group 1 was castrated on the 78th day, grous 2,3,4,5 were immunized against LHRH-BSA conjugate with Freund adjuvant(FA) following different vaccination schedules, group 6 served as control which was immunized ag￣ainst BSA with FA. This trial showed that, in contrast with BSA control, the level of serum testosterone decreased greatly in both immunized groups 4,5 and castrated group, the testis, combs and wattles of cockerels in each immunized group became atrophic to varying degree, development of seminiferous tubules were inhibited and the tubules were abnormal, immunization on the 40th or 20th day significantly brought better castration effects than immunization on the 60th day ,two immunizations resulted in better immunization effect than one immunization though the same dose of antigen was used, satisfactory immunocastration effects can be achieved in cockerels by immunization with LHRH-BSA in FA on the 20th and 40th day respectively.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第2期76-81,共6页
Journal of South China Agricultural University
关键词
鸡
公鸡
免疫去势
LHRH
luteinizing hormone releasing hormone (LHRH)
cockerel immunocastration
